Crumbs3 regulates the expression of glycosphingolipids on the plasma membrane to promote colon cancer cell migration

被引：8
作者：
Iioka, Hidekazu [1 ]
Saito, Ken [1 ]
Kondo, Eisaku [1 ]
机构：
[1] Niigata Univ, Grad Sch Med & Dent Sci, Div Mol & Cellular Pathol, Niigata, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2019年 / 519卷 / 02期
基金：
日本学术振兴会;
关键词：
Cell polarity; Crumbs3; Ganglioside GM3; Cell migration; Metastasis; POLARITY DETERMINANT CRUMBS; GROWTH-FACTOR RECEPTOR; GM3; GANGLIOSIDE; TUMOR; MOTILITY; APOPTOSIS; ADHESION; CD9;
D O I：
10.1016/j.bbrc.2019.08.161
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cell polarity regulator Crumbs3 (Crb3) promotes colon cancer cell migration and metastasis. However, the underlying mechanism of cancer cell migration regulated by Crb3 has not been fully elucidated. Here, we demonstrated that Crb3 is associated with cell migration by regulating glycosphingolipid (GSL) expression in human colon cancer cells. Crb3-knockout (KO) cells showed a remarkable increase in ganglioside GM3 (GM3) on the cell surface. Reduced migration by Crb3-KO cells was restored by forced expression of both Crb3 and Neuraminidase3 (Neu3). Immunofluorescent staining revealed that most Crb3 is colocalized with the recycling endosome marker Rab11. These findings show that Crb3 may promote colon cancer cell migration by regulating the expression of GSLs on the cell surface. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码：287 / 293
页数：7
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