Synthetic biology: New strategies for directing design

被引：28

作者：

Lynch, Sean A. ^{[1
]}

Gill, Ryan T. ^{[1
]}

机构：

[1] Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80309 USA

来源：

METABOLIC ENGINEERING | 2012年 / 14卷 / 03期

基金：

美国国家科学基金会;

关键词：

Genome engineering; Directed evolution; Synthetic biology; Recombineering; ESCHERICHIA-COLI; GENE-EXPRESSION; TRANSCRIPTION MACHINERY; IN-VITRO; DNA; RECOMBINATION; EVOLUTION; MUTANTS; YEAST; PATHWAY;

D O I：

10.1016/j.ymben.2011.12.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The advancement of synthetic biology is thanks, in large part, to continuing improvements in DNA synthesis. The expansion of synthetic biology into the realm of metabolic engineering has shifted the focus from simply making novel synthetic biological parts to answering the question of how we employ these biological parts to construct genomes that ultimately give rise to useful phenotypes. Much like protein engineering, the answer to this will be arrived at following the combination of rational design and evolutionary approaches. This review will highlight some of the new DNA synthesis-enabled search methods and discuss the application of such methods to the creation of synthetic gene networks and genomes. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：205 / 211

页数：7

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