Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex

被引：8

作者：

Alasaad, Samer ^{[1
,2
]}

Biebach, Iris ^{[2
]}

Grossen, Christine ^{[2
,3
]}

Soriguer, Ramon C. ^{[1
]}

Perez, Jesus M. ^{[4
]}

Keller, Lukas F. ^{[2
]}

机构：

[1] CSIC, Estn Biol Donana, Seville 41092, Spain

[2] Univ Zurich, Inst Evolutionary Biol & Environm Studies IEU, CH-8057 Zurich, Switzerland

[3] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland

[4] Univ Jaen, Dept Biol Anim Biol Vegetal & Ecol, Jaen 23071, Spain

来源：

EUROPEAN JOURNAL OF WILDLIFE RESEARCH | 2012年 / 58卷 / 04期

关键词：

Capra ibex; Capra pyrenaica; Goats; OLADRB1; MHC DRB1; Linkage disequilibrium; DRB-STR method; MAJOR HISTOCOMPATIBILITY COMPLEX; INTRONIC MICROSATELLITE; BALANCING SELECTION; SEQUENCE-ANALYSIS; SPANISH IBEX; ALLELES; POLYMORPHISM; DIVERSITY; EVOLUTION; PERSPECTIVE;

D O I：

10.1007/s10344-011-0592-0

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

In an analysis of a microsatellite locus (OLADRB1) linked to the MHC DRB1 gene of Iberian and Alpine ibex (Capra pyrenaica and Carpa ibex), we detected strong linkage disequilibrium between both loci. The allele length polymorphism at OLADRB1 was unambiguously linked to a particular DRB1 allele. This allowed us to develop a DRB-STR matching method for both ibex species. Validation of the DRB-STR matching method was performed in 160 Iberian ibex from Spain and 98 Alpine ibex from Switzerland and Italy. This simple and relatively inexpensive protocol may find wide applications in a variety of research areas (e.g., mate choice, pathogen-driven selection) and in the biological conservation and management of the Western European ibex populations.

引用

页码：743 / 748

页数：6

共 50 条

[1] Low diversity in the major histocompatibility complex class II DRB1 gene of the Spanish ibex, Capra pyrenaica [J].

Amills, M ;

Jiménez, N ;

Jordana, J ;

Riccardi, A ;

Fernández-Arias, A ;

Guiral, J ;

Bouzat, JL ;

Folch, J ;

Sànchez, A .

HEREDITY, 2004, 93 (03) :266-272

[2] A PCR-RFLP typing method for the caprine Mhc class II DRB gene [J].

Amills, M ;

Francino, O ;

Sanchez, A .

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 55 (1-3) :255-260

[3] New generation sequencers as a tool for genotyping of highly polymorphic multilocus MHC system [J].

Babik, Wieslaw ;

Taberlet, Pierre ;

Ejsmond, Maciej Jan ;

Radwan, Jacek .

MOLECULAR ECOLOGY RESOURCES, 2009, 9 (03) :713-719

[4] Tracing the origin of HLA-DRB1 alleles by microsatellite polymorphism [J].

Bergström, TF ;

Engkvist, H ;

Erlandsson, R ;

Josefsson, A ;

Mack, SJ ;

Erlich, HA ;

Gyllensten, U .

AMERICAN JOURNAL OF HUMAN GENETICS, 1999, 64 (06) :1709-1718

[5] MHC studies in nonmodel vertebrates: what have we learned about natural selection in 15 years? [J].

Bernatchez, L ;

Landry, C .

JOURNAL OF EVOLUTIONARY BIOLOGY, 2003, 16 (03) :363-377

[6] A strong genetic footprint of the re-introduction history of Alpine ibex (Capra ibex ibex) [J].

Biebach, Iris ;

Keller, Lukas F. .

MOLECULAR ECOLOGY, 2009, 18 (24) :5046-5058

[7]

Botani L, 2003, FAUNA ITALIA MAMMALI, P434

[8]

Cabrera A, 1911, P ZOOL SOC LOND, V1911, P963

[9]

Cabrera A., 1914, FAUNA IBERICA MAMIFE

[10]

Couturier M.A.J, 1962, BOUQUETIN ALPES CAPR, P1564

← 1 2 3 4 5 →