Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos

被引：5
作者：
Pradiee, J. [1 ,2 ]
Cardoso, T. F. [1 ,3 ]
Silva, E. F. [1 ,3 ]
Goncalves, A. O. [1 ,2 ]
Gastal, G. D. A. [1 ,2 ]
Rosa, C. E. [6 ]
Mondadori, R. G. [1 ,4 ]
Pegoraro, L. M. C. [5 ]
Vieira, A. D. [1 ,2 ]
Lucia, T., Jr. [1 ,2 ]
机构：
[1] ReproPel, Capao Do Leao, RS, Brazil
[2] Fac Vet, Pelotas, RS, Brazil
[3] Ctr Desenvolvimento Tecnol, Pelotas, RS, Brazil
[4] Univ Fed Pelotas, Inst Biol, Pelotas, RS, Brazil
[5] Embrapa Clima Temperado, Pelotas, RS, Brazil
[6] Univ Fed Rio Grande, Inst Ciencias Biol, Rio Grande, RS, Brazil
来源：
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2016年 / 68卷 / 05期
关键词：
sheep embryos; antioxidant; reactive oxygen species; frozen ram sperm; vitrification; IN-VITRO; REDUCED GLUTATHIONE; BOAR SPERM; 2-MERCAPTOETHANOL; CRYOPRESERVATION; SPERMATOZOA; PARAMETERS; FERTILITY; INTEGRITY; MOTILITY;
D O I：
10.1590/1678-4162-8479
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The effects of beta-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50 mu M BME and 600 mu M cysteine (64.3% and 36.6%, respectively). Post-thawing viability of vitrified embryos was similar between media (P>0.05). Cysteine and BME had no influence on the post-thawing viability and oxidative activity of ram sperm and on the viability of vitrified sheep embryos.
引用
收藏
页码：1309 / 1315
页数：7
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