Quantification of myrislignan in rat plasma by solid-phase extraction and reversed-phase high-performance liquid chromatography

A rapid and simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for determination of myrislignan in rat plasma after intravenous administration. The analytes extracted from plasma samples by solid-phase extraction were successfully carried out on a Diamonsil (TM) ODS C-18 column (250 x 4.6 mm i.d., 5 mu m) with an RP18 guard column (8 x 4.6 mm id., 5 mu m) and a mobile phase of MeOH-H2O (4:1, v/v). The UV detector was set at a single wave-length of 270 nm. The linear ranges of the standard curves were 0.5-30.0 mu g/mL with the correlation coefficients greater than 0.9992. The lower limits of detection and quantification were 0.1 and 0.3 mu g/mL for myrislignan. Intra- and inter-day precisions were 2.4-7.5 and 1.3-5.7%, respectively. The extraction recovery from plasma was more than 90%. This assay method has been successfully used to study the pharmacokinetics of myrislignan in rats. Copyright (C) 2008 John Wiley & Sons, Ltd.