Effects and mechanism of cerebroprotein hydrolysate on learning and memory ability in mice

被引：13

作者：

An, L. ^{[1
]}

Han, X. ^{[1
]}

Li, H. ^{[1
]}

Ma, Y. ^{[1
]}

Shi, L. ^{[1
]}

Xu, G. ^{[1
]}

Yuan, G. ^{[1
]}

Sun, J. ^{[1
]}

Zhao, N. ^{[1
]}

Sheng, Y. ^{[1
]}

Wang, M. ^{[1
]}

Du, P. ^{[1
]}

机构：

[1] Beihua Univ, Coll Pharm, Jilin, Jilin, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2016年 / 15卷 / 03期

关键词：

Cerebroprotein hydrolysate; Learning; Memory; Free radical injury; OXIDATIVE STRESS; IMPAIRMENT; PEPTIDES; PROTECTS; BRAIN;

D O I：

10.4238/gmr.15038804

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cerebroprotein hydrolysate is an extract from porcine brain tissue that acts on the central nervous system in various ways to protect neurons and improve memory, attention, and vigilance. This study examined the effect and mechanism of cerebroprotein hydrolysate on learning and memory in mice with scopolamine induced impairment. Mice were given an intraperitoneal injection of scopolamine hydrobromide to establish a murine model of learning and memory impairment. After 35 successive days of cerebroprotein hydrolysate treatment, their behaviors were observed in the Morris water maze and step-down test. Superoxide dismutase (SOD), Na+-K+-ATPase, and acetylcholinesterase (AChE) activity, and malondialdehyde (MDA), gamma-aminobutyric acid (GABA), and glutamic acid (Glu) levels in the brain tissue of the mice were determined, and pathological changes in the hippocampus were examined. The results of the water-maze test showed that cerebroprotein hydrolysate shortened the escape latency and increased the number of platform crossings. In the step-down test, cerebroprotein hydrolysate treatment prolonged the step-down latency and reduced the number of errors; cerebroprotein hydrolysate increased the activity of SOD, Na+-K+-ATPase, and AChE, reduced the levels of MDA, decreased the Glu/GABA ratio in brain tissue, and reduced pathological changes in the hippocampus. The results indicate that cerebroprotein hydrolysate can improve learning and memory in mice with scopolamine-induced impairment. This effect may be associated with its ability to reduce injury caused by free radicals, improve acetylcholine function, and modulate the Glu/GABA learning and memory regulation system, reducing excitotoxicity caused by Glu.

引用

页数：10

共 21 条

[1] Toxic effects of perinatal lead exposure on the brain of rats:: Involvement of oxidative stress and the beneficial role of antioxidants [J].