Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydrolase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg

被引：15

作者：

Pedrosa Ribeiro Mendes, Rita Gabriela ^{[1
]}

do Nascimento Gusmao, Michelia Antonia ^{[1
]}

Ribeiro Comes Maia, Ana Carolina ^{[1
]}

Detoni, Michelle de Lima ^{[1
]}

Porcino, Gabriane Nascimento ^{[1
]}

Soares, Thais Vieira ^{[1
]}

Juliano, Maria Aparecida ^{[2
]}

Juliano, Luiz ^{[2
]}

Zech Coelho, Paulo Marcos ^{[3
]}

Lenzi, Henrique Leonel ^{[4
]}

Faria-Pinto, Priscila ^{[1
]}

Vasconcelos, Eveline Gomes ^{[1
]}

机构：

[1] Univ Fed Juiz de Fora, Dept Bioquim, Inst Ciencias Biol, Juiz De Fora, MG, Brazil

[2] Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, Sao Paulo, Brazil

[3] Inst Pesquisas Rene Rachou Fiocruz, Belo Horizonte, MG, Brazil

[4] Inst Oswaldo Cruz Fiocruz, Lab Patol, Rio De Janeiro, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2011年 / 106卷 / 07期

关键词：

Schistosoma mansoni; NTPDase; potato apyrase; peptide; POTATO APYRASE; IMMUNE-RESPONSES; LOCALIZATION; CERCARIAE; SURFACE; IGG1;

D O I：

10.1590/S0074-02762011000700005

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs.

引用

页码：808 / 813

页数：6