Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

被引：31

作者：

Chen, Bing-Hung ^{[1
]}

Hung, Mei-Huei ^{[1
]}

Chen, Jeff Yi-Fu ^{[1
]}

Chang, Hsueh-Wei ^{[2
]}

Yu, Meng-Lung ^{[2
]}

Wan, Lei ^{[3
]}

Tsai, Fuu Jen ^{[3
]}

Wang, Tzu-Pin ^{[4
]}

Fu, Tzu-Fun ^{[5
]}

Chiu, Chien-Chih ^{[1
]}

机构：

[1] Kaohsiung Med Univ, Dept Biotechnol, Kaohsiung 807, Taiwan

[2] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Ctr Excellence Environm Med,Canc Ctr, Dept Biomed Sci & Environm Biol,Grad Inst Nat Pro, Kaohsiung 807, Taiwan

[3] China Med Univ Hosp, Grad Inst Chinese Med Sci, Dept Med Genet & Med Res, Taichung, Taiwan

[4] Kaohsiung Med Univ, Dept Med & Appl Chem, Kaohsiung, Taiwan

[5] Natl Cheng Kung Univ, Dept Med Lab Sci & Biotechnol, Tainan 70101, Taiwan

来源：

FOOD CHEMISTRY | 2012年 / 132卷 / 02期

关键词：

Allergy; Degranulation; Grapeseed extract; High-affinity IgE receptor; Mast cell; FC-EPSILON-RI; SEED EXTRACT; ANTIGEN; INHIBITION; CONSTITUENTS; MECHANISMS; RECEPTORS; CALCIUM; GROWTH; IH636;

D O I：

10.1016/j.foodchem.2011.11.079

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 mu g/ml) reduced IgE-antigen mediated release of beta-hexosaminidase and histamine in RBL-2H3 cells. Additionally. GSE reversibly inhibited expression of Fc epsilon RI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca2+ influx upon antigen stimulation was inhibited. Suppression on Fc epsilon RI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：968 / 974

页数：7

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