DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients

被引：7
|
作者：
Pereira Lima, Patricia Santos [1 ,2 ]
Molffeta, Greice Andreoti
de Araujo, Amelia Goes [3 ,4 ,5 ]
Zago, Marco Antonio [3 ,4 ,5 ]
da Silva, Wilson Araujo, Jr. [4 ,5 ]
机构：
[1] Univ Sao Paulo, Lab Genet Mol & Bioinformat, Fac Med Ribeirao Preto, Dept Genet,Fundacao Hemoctr Ribeirao Preto, BR-14051140 Ribeirao Preto, SP, Brazil
[2] Univ Estadual Sudoeste Bahia, Dept Ciencias Nat, Vitoria Da Conquista, BA, Brazil
[3] Univ Sao Paulo, Dept Clin Med, Fac Med Ribeirao Preto, BR-14051140 Ribeirao Preto, SP, Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Terapia Celular, BR-14051140 Ribeirao Preto, SP, Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Reg Hemoterapia, BR-14051140 Ribeirao Preto, SP, Brazil
来源：
GENETICS AND MOLECULAR BIOLOGY | 2008年 / 31卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
CpG island; DNA methylation; CDKN2B;
D O I：
10.1590/S1415-47572008000400005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.
引用
收藏
页码：632 / 638
页数：7
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