1β-Hydroxy-2-oxopomolic Acid Isolated from Agrimonia pilosa Extract Inhibits Adipogenesis in 3T3-L1 Cells

被引：18
作者：
Ahn, Eun-Kyung [1 ]
Lee, Jung A. [1 ]
Seo, Dong-Wan [2 ]
Hong, Seong Su [1 ]
Oh, Joa Sub [1 ,2 ]
机构：
[1] Gyeonggi Inst Sci & Technol Promot, Inst Nat Prod Res, Suwon 443766, Gyeonggi Do, South Korea
[2] Dankook Univ, Coll Pharm, Cheonan Si 330714, South Korea
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2012年 / 35卷 / 05期
关键词：
1 beta-hydroxy-2-oxopomolic acid; adipogenesis; adipogenic marker gene; 3T3-L1 cell line; REVERSES INSULIN-RESISTANCE; ADIPOCYTE DIFFERENTIATION; PPAR-GAMMA; GENE-EXPRESSION; ENERGY-BALANCE; C/EBP-ALPHA; TRANSCRIPTION; ADIPONECTIN; GLUCOSE; OBESITY;
D O I：
10.1248/bpb.35.643
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In order to determine anti-adipogenic effect, this study investigated 1 beta-hydroxy-2-oxopomolic acid (HOA) isolated from Agrimonia pilosa inhibits adipocyte differentiation and expression of adipogenic marker genes, such as peroxisome proliferator activated receptor gamma (PPAR gamma), CCAAT-enhancer-binding protein alpha (C/EBP alpha), glucose transporter 4 (GLUT4), adiponectin, adipocyte fatty acid-binding protein 2 (aP2), adipocyte determination and differentiation factor 1/sterol regulatory element binding protein 1c (ADD1/SREBP1c), resistin, and fatty acid synthase (Fas) in 3T3-L1 preadipocyte. We demonstrated that HOA induced a significant decrease in lipid accumulation and expression of adipogenic marker genes in a dose-dependent manner. In addition, HOA reduced the transcripitional activity of PPAR gamma induced by troglitazone, a potent diabetes agent; it also suppressed expression of PPAR gamma and C/EBP alpha protein levels. Our data suggest that HOA isolated from Agrimonia pilosa inhibits adipocyte differentiation through downregulation of various adipocytokines by blocking PPAR gamma and C/EBP alpha expression.
引用
收藏
页码：643 / 649
页数：7
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