1β-Hydroxy-2-oxopomolic Acid Isolated from Agrimonia pilosa Extract Inhibits Adipogenesis in 3T3-L1 Cells

被引：18
作者：
Ahn, Eun-Kyung [1 ]
Lee, Jung A. [1 ]
Seo, Dong-Wan [2 ]
Hong, Seong Su [1 ]
Oh, Joa Sub [1 ,2 ]
机构：
[1] Gyeonggi Inst Sci & Technol Promot, Inst Nat Prod Res, Suwon 443766, Gyeonggi Do, South Korea
[2] Dankook Univ, Coll Pharm, Cheonan Si 330714, South Korea
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2012年 / 35卷 / 05期
关键词：
1 beta-hydroxy-2-oxopomolic acid; adipogenesis; adipogenic marker gene; 3T3-L1 cell line; REVERSES INSULIN-RESISTANCE; ADIPOCYTE DIFFERENTIATION; PPAR-GAMMA; GENE-EXPRESSION; ENERGY-BALANCE; C/EBP-ALPHA; TRANSCRIPTION; ADIPONECTIN; GLUCOSE; OBESITY;
D O I：
10.1248/bpb.35.643
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In order to determine anti-adipogenic effect, this study investigated 1 beta-hydroxy-2-oxopomolic acid (HOA) isolated from Agrimonia pilosa inhibits adipocyte differentiation and expression of adipogenic marker genes, such as peroxisome proliferator activated receptor gamma (PPAR gamma), CCAAT-enhancer-binding protein alpha (C/EBP alpha), glucose transporter 4 (GLUT4), adiponectin, adipocyte fatty acid-binding protein 2 (aP2), adipocyte determination and differentiation factor 1/sterol regulatory element binding protein 1c (ADD1/SREBP1c), resistin, and fatty acid synthase (Fas) in 3T3-L1 preadipocyte. We demonstrated that HOA induced a significant decrease in lipid accumulation and expression of adipogenic marker genes in a dose-dependent manner. In addition, HOA reduced the transcripitional activity of PPAR gamma induced by troglitazone, a potent diabetes agent; it also suppressed expression of PPAR gamma and C/EBP alpha protein levels. Our data suggest that HOA isolated from Agrimonia pilosa inhibits adipocyte differentiation through downregulation of various adipocytokines by blocking PPAR gamma and C/EBP alpha expression.
引用
收藏
页码：643 / 649
页数：7
相关论文
共 34 条
[1]   Regulation of fasted blood glucose by resistin [J].
Banerjee, RR ;
Rangwala, SM ;
Shapiro, JS ;
Rich, AS ;
Rhoades, B ;
Qi, Y ;
Wang, J ;
Rajala, MW ;
Pocai, A ;
Scherer, PE ;
Steppan, CM ;
Ahima, RS ;
Obici, S ;
Rossetti, L ;
Lazar, MA .
SCIENCE, 2004, 303 (5661) :1195-1198
[2]   Adipocyte differentiation: A transcriptional regulatory cascade [J].
Brun, RP ;
Kim, JB ;
Hu, E ;
Altiok, S ;
Spiegelman, BM .
CURRENT OPINION IN CELL BIOLOGY, 1996, 8 (06) :826-832
[3]   Adipose-specific overexpression of GLUT4 reverses insulin resistance and diabetes in mice lacking GLUT4 selectively in muscle [J].
Carvalho, E ;
Kotani, K ;
Peroni, OD ;
Kahn, BB .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2005, 289 (04) :E551-E561
[4]   Regulation of adipocytokines and insulin resistance [J].
Fasshauer, M ;
Paschke, R .
DIABETOLOGIA, 2003, 46 (12) :1594-1603
[5]   Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARγ2 and C/EBPα in 3T3-L1 cells [J].
Furuyashiki, T ;
Nagayasu, H ;
Aoki, Y ;
Bessho, H ;
Hashimoto, T ;
Kanazawa, K ;
Ashida, H .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2004, 68 (11) :2353-2359
[6]  
안인파, 2005, Natural Product Sciences, V11, P196
[7]   FATTY-ACIDS IN CELL SIGNALING - MODULATION BY LIPID-BINDING PROTEINS [J].
GLATZ, JFC ;
BORCHERS, T ;
SPENER, F ;
VANDERVUSSE, GJ .
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1995, 52 (2-3) :121-127
[8]   Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis [J].
Harmon, AW ;
Harp, JB .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2001, 280 (04) :C807-C813
[9]   Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARγ pathway [J].
Huang, Cheng ;
Zhang, Yuebo ;
Gong, Zhenwei ;
Sheng, Xiaoyan ;
Li, Zongmeng ;
Zhang, Wei ;
Qin, Ying .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 348 (02) :571-578
[10]   Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells [J].
Jeon, T ;
Hwang, SG ;
Hirai, S ;
Matsui, T ;
Yano, H ;
Kawada, T ;
Lim, BO ;
Park, DK .
LIFE SCIENCES, 2004, 75 (26) :3195-3203
1234