Low Molecular Weight Polyethylenimine-graft-Tween 85 for Effective Gene Delivery: Synthesis and in Vitro Characteristics

被引：14

作者：

Xiao, Jisheng ^{[1
]}

Duan, Xiaopin ^{[1
,2
]}

Yin, Qi ^{[1
]}

Chen, Lingli ^{[1
]}

Zhang, Zhiwen ^{[1
]}

Li, Yaping ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China

[2] Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China

来源：

BIOCONJUGATE CHEMISTRY | 2012年 / 23卷 / 02期

基金：

中国国家自然科学基金;

关键词：

TRANSFECTION EFFICIENCY; VECTORS; DNA; NANOPARTICLES; PEI; DERIVATIVES; COPOLYMERS; GLYCOL); DESIGN; LIPIDS;

D O I：

10.1021/bc200504v

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of safe and efficient gene delivery systems is still a challenge for successful gene therapy. In this work, low molecular weight polyethylenimine (PEI 2K) was modified by Tween 85, which bears three oleate chains. Tween 85 modified PEI 2K (TP) could condense DNA efficiently, and TP/DNA complexes (TPCs) showed high resistance to salt-induced aggregation and enzymatic degradation. In addition, TP did not show the obvious cytotoxicity. The introduction of Tween 85 led to a significant increase in the cellular uptake of complexes with higher transfection efficiency, which was strongly inhibited by the addition of free Tween 85 in MCF-7/ADR cells, but not in MCF-7 cells. These results indicated that TP could be a potentially safe and effective copolymer for gene delivery, and TPCs could be taken up mainly by Tween 85-mediated endocytosis in MCF-7/ADR cells.

引用

页码：222 / 231

页数：10

共 34 条

[1] Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis [J].

Akinc, A ;

Thomas, M ;

Klibanov, AM ;

Langer, R .

JOURNAL OF GENE MEDICINE, 2005, 7 (05) :657-663

[2] Formulation and Delivery of siRNA by Oleic Acid and Stearic Acid Modified Polyethylenimine [J].

Alshamsan, Aws ;

Haddadi, Azita ;

Incani, Vanessa ;

Samuel, John ;

Lavasanifar, Afsaneh ;

Uludag, Hasan .

MOLECULAR PHARMACEUTICS, 2009, 6 (01) :121-133

[3] Synthesis and gene transfection efficacies of PEI-cholesterol-based lipopolymers [J].

Bajaj, Avinash ;

Kondaiah, Paturu ;

Bhattacharya, Santanu .

BIOCONJUGATE CHEMISTRY, 2008, 19 (08) :1640-1651

[4] Structure-activity investigation on the gene transfection properties of cardiolipin mimicking gemini lipid analogues [J].

Bajaj, Avinash ;

Paul, Bishwajit ;

Kondaiah, Paturu ;

Bhattacharya, Santanu .

BIOCONJUGATE CHEMISTRY, 2008, 19 (06) :1283-1300

[5] Synthesis and gene transfer activities of novel serum compatible cholesterol-based gemini lipilds possessing oxyethylene-type spacers [J].

Bajaj, Avinash ;

Kondaiah, Paturu ;

Bhattacharya, Santanu .

BIOCONJUGATE CHEMISTRY, 2007, 18 (05) :1537-1546

[6] Cationic oxyethylene lipids. Synthesis, aggregation, and transfection properties [J].

Bhattacharya, S ;

Dileep, PV .

BIOCONJUGATE CHEMISTRY, 2004, 15 (03) :508-519

[7] Advances in gene delivery through molecular design of cationic lipids [J].

Bhattacharya, Santanu ;

Bajaj, Avinash .

CHEMICAL COMMUNICATIONS, 2009, (31) :4632-4656

[8] Targeted Gene Delivery Mediated by Folate-polyethylenimine-block-poly(ethylene glycol) with Receptor Selectivity [J].

Cheng, Han ;

Zhu, Jing-Ling ;

Zeng, Xuan ;

Jing, Yue ;

Zhang, Xian-Zheng ;

Zhuo, Ren-Xi .

BIOCONJUGATE CHEMISTRY, 2009, 20 (03) :481-487

[9] Polyethylenimine-grafted copolymer of poly(L-lysine) and poly(ethylene glycol) for gene delivery [J].

Dai, Jian ;

Zou, Seyin ;

Pei, Yuanyuan ;

Cheng, Du ;

Ai, Hua ;

Shuai, Xintao .

BIOMATERIALS, 2011, 32 (06) :1694-1705

[10] Incorporation of oxyethylene units between hydrocarbon chain and pseudoglyceryl backbone in cationic lipid potentiates gene transfection efficiency in the presence of serum [J].

Dileep, PV ;

Antony, A ;

Bhattacharya, S .

FEBS LETTERS, 2001, 509 (02) :327-331

← 1 2 3 4 →