Identification of a new hobo element in the cabbage moth, Mamestra brassicae (Lepidoptera)

被引：6

作者：

Borsatti, F ^{[1
]}

Azzoni, P ^{[1
]}

Mandrioli, M ^{[1
]}

机构：

[1] Univ Modena & Reggio Emilia, Dipartimento Biol Anim, IT-41100 Modena, Italy

来源：

HEREDITAS | 2003年 / 139卷 / 02期

关键词：

D O I：

10.1111/j.1601-5223.2003.01768.x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A complete hobo-like element, called Mbhobo, was identified in the cabbage moth, Mamestra brassicae. This element has a high sequence similarity to the HFL1 hobo element of Drosophila melanogaster. Amplification of Mbhobo termini indicated that transposition occurred into a 5'-GTGGGTAC-3' target sequence that was duplicated upon insertion. This target site conforms to the consensus sequence established for the insertion sites of insect hAT elements. Mbhobo has a single 1935 bp long ORF with significant homology to the D. melanogaster HFL1 hobo transposase. FISH experiments evidenced Mbhobo clusters located in heterochromatic regions of Z and W sex chromosomes and in heterochromatic areas of chromosome pair 10.

引用

页码：151 / 155

页数：5

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