Biochip for astrobiological applications: Investigation of low energy protons effects on antibody performances

被引：19

作者：

Baque, M. ^{[1
]}

Le Postollec, A. ^{[2
,3
]}

Coussot, G. ^{[1
]}

Moreau, T. ^{[1
]}

Desvignes, I. ^{[1
]}

Incerti, S. ^{[4
]}

Moretto, P. ^{[4
]}

Dobrijevic, M. ^{[2
,3
]}

Vandenabeele-Trambouze, O. ^{[1
]}

机构：

[1] Univ Montpellier 2, Univ Montpellier 1, CNRS, IBMM,UMR 5247, F-34095 Montpellier 5, France

[2] Univ Bordeaux, LAB, Bordeaux, France

[3] CNRS, INSU, UMR 5804, F-33271 Floirac, France

[4] Univ Bordeaux, CNRS, IN2P3, CENBG,UMR 5797, Gradignan, France

来源：

PLANETARY AND SPACE SCIENCE | 2011年 / 59卷 / 13期

关键词：

Antibody-based microarrays; Cosmic radiation; Life-detection instrument; Planetary exploration; PROTEIN STABILIZATION; LIFE; SIMULATION; STABILITY; IMMOBILIZATION; MICROARRAYS; STRATEGIES; MECHANISM;

D O I：

10.1016/j.pss.2011.06.009

中图分类号：

P1 [天文学];

学科分类号：

0704 ;

摘要：

Antibody-based micro-arrays instruments are very promising tools for the search for biomarkers in planetary exploration missions. Since such instruments have never been used in this context, it is important to test their resistance to space constraints. In particular, cosmic particles might be deleterious. In the present study, we have investigated the effect of low energy protons (2 MeV) on antibody performances with fluences levels much greater than expected for a typical mission to Mars. We show that these particles do not alter significantly the antibody recognition capability for both free (in solution) and grafted (covalently bound to the support) freeze-dried antibodies. Details of the freeze-dried drying process used to optimize antibody performances during our experiments are also presented. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1490 / 1497

页数：8

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