Biocontrol potential and polyphasic characterization of novel native Trichoderma strains against Macrophomina phaseolina isolated from sorghum and common bean

被引：29

作者：

Larralde-Corona, C. P. ^{[1
]}

Santiago-Mena, M. R. ^{[1
]}

Sifuentes-Rincon, A. M. ^{[1
]}

Rodriguez-Luna, I. C. ^{[1
]}

Rodriguez-Perez, M. A. ^{[1
]}

Shirai, K. ^{[2
]}

Narvaez-Zapata, J. A. ^{[1
]}

机构：

[1] Inst Politecn Nacl, Ctr Biotecnol Genom, Reynosa 88710, Tamaulipas, Mexico

[2] Univ Autonoma Metropolitana Iztapalapa, Dept Biotecnol, Iztapalapa, Mexico

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2008年 / 80卷 / 01期

关键词：

biocontrol; Trichoderma isolates; Macrophomina phaseolina; polyphasic characterization;

D O I：

10.1007/s00253-008-1532-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Native strains of Trichoderma isolated from sorghum and common bean crop soils were investigated to assess their biocontrol potential over the phytopathogenic fungus Macrophomina phaseolina, isolated from diseased plants. The Trichoderma strains were characterized with a polyphasic approach, which combined the analysis of their morphological characteristics, enzymatic activity, macro- and microculture test results, rDNA restriction patterns (AFLP), ITS1-5.8S-ITS2 rDNA sequences, and protein profiles. The integration of these data sets can be used to select new isolates as biological control agents against native fungal phytopathogens. In general, we observed a positive correlation between the secretion of beta-1,3-glucanase and N-acetylhexosaminidase, and the biocontrol capacities of all the Trichoderma isolates. Strains with the best hyperparasitic behavior against M. phaseolina isolated from diseased bean and sorghum were Trichoderma sp. (TCBG-2) and Trichoderma koningiopsis (TCBG-8), respectively.

引用

页码：167 / 177

页数：11

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