Toxicity and Internalization of CuO Nanoparticles to Prokaryotic Alga Microcystis aeruginosa as Affected by Dissolved Organic Matter

被引:319
作者
Wang, Zhenyu [1 ,2 ]
Li, Jing [2 ]
Zhao, Jian [1 ,2 ]
Xing, Baoshan [1 ]
机构
[1] Univ Massachusetts, Dept Plant Soil & Insect Sci, Amherst, MA 01003 USA
[2] Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China
基金
中国国家自然科学基金;
关键词
BULK ZNO; PSEUDOKIRCHNERIELLA-SUBCAPITATA; GROWTH-INHIBITION; OXIDATIVE STRESS; CARBON NANOTUBES; COPPER TOXICITY; TIO2; CYTOTOXICITY; ECOTOXICITY; ADSORPTION;
D O I
10.1021/es2010573
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
This is the first study investigating the toxicity of nanoparticles (NPs) to algae in the presence of dissolved organic matter (DOM). Suwannee river fulvic acid (SRFA), a type of DOM, could significantly increase the toxicity of CuO NPs to prokaryotic alga Microcystis aeruginosa. Internalization of CuO NPs was observed for the first time in the intact algal cells using high resolution transmission electron microscopy (HRTEM), and the cell uptake was enhanced by SRFA. A fast Fourier transformation (FFT)/inversed FFT (IFFT) process revealed that a main form of intracellular NPs was Cu2O, and an intracellular environment may reduce CuO into Cu2O. The internalization behavior alone did not seem to pose a hazard to membrane integrity as shown from the flow cytometry data. Elevated CuO nanotoxicity by SRFA was related to a combination of a lesser degree of aggregation, higher Cu2+ release, and enhanced internalization of CuO NPs.
引用
收藏
页码:6032 / 6040
页数:9
相关论文
共 42 条
[1]   Toxicity of nanoparticles of CuO, ZnO and TiO2 to microalgae Pseudokirchneriella subcapitata [J].
Aruoja, Villem ;
Dubourguier, Henri-Charles ;
Kasemets, Kaja ;
Kahru, Anne .
SCIENCE OF THE TOTAL ENVIRONMENT, 2009, 407 (04) :1461-1468
[2]   Surface-Charge-Dependent Cell Localization and Cytotoxicity of Cerium Oxide Nanoparticles [J].
Asati, Atul ;
Santra, Santimukul ;
Kaittanis, Charalambos ;
Perez, J. Manuel .
ACS NANO, 2010, 4 (09) :5321-5331
[3]   Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells [J].
AshaRani, P. V. ;
Mun, Grace Low Kah ;
Hande, Manoor Prakash ;
Valiyaveettil, Suresh .
ACS NANO, 2009, 3 (02) :279-290
[4]   Development and field validation of a predictive copper toxicity model for the green alga Pseudokirchneriella subcapitata [J].
De Schamphelaere, KAC ;
Vasconcelos, FM ;
Heijerick, DG ;
Tack, FMG ;
Delbeke, K ;
Allen, HE ;
Janssen, CR .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2003, 22 (10) :2454-2465
[5]   Bioavailability models for predicting copper toxicity to freshwater green microalgae as a function of water chemistry [J].
De Schamphelaere, Karel A. C. ;
Janssen, Colin R. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2006, 40 (14) :4514-4522
[6]   Silver Nanoparticle Impact on Bacterial Growth: Effect of pH, Concentration, and Organic Matter [J].
Fabrega, Julia ;
Fawcett, Shona R. ;
Renshaw, Joanna C. ;
Lead, Jamie R. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2009, 43 (19) :7285-7290
[7]   Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapitata):: The importance of particle solubility [J].
Franklin, Natasha M. ;
Rogers, Nicola J. ;
Apte, Simon C. ;
Batley, Graeme E. ;
Gadd, Gerald E. ;
Casey, Philip S. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2007, 41 (24) :8484-8490
[8]   pH-dependent toxicity of copper and uranium to a tropical freshwater alga (Chlorella sp.) [J].
Franklin, NM ;
Stauber, JL ;
Markich, SJ ;
Lim, RP .
AQUATIC TOXICOLOGY, 2000, 48 (2-3) :275-289
[9]   Effect of selenate on growth and photosynthesis of Chlamydomonas reinhardtii [J].
Geoffroy, Laure ;
Gilbin, Rodolphe ;
Simon, Olivier ;
Floriani, Magali ;
Adam, Christelle ;
Pradines, Catherine ;
Cournac, Laurent ;
Garnier-Laplace, Jacqueline .
AQUATIC TOXICOLOGY, 2007, 83 (02) :149-158
[10]   Colloidal Stability of Al2O3 Nanoparticles as Affected by Coating of Structurally Different Humic Acids [J].
Ghosh, Saikat ;
Mashayekhi, Hamid ;
Bhowmik, Prasanta ;
Xing, Baoshan .
LANGMUIR, 2010, 26 (02) :873-879