The effects of dsRNA mycoviruses on growth and murine virulence of Aspergillus fumigatus

被引：69

作者：

Bhatti, Muhammad F. ^{[1
]}

Jamal, Atif ^{[1
,2
]}

Petrou, Michael A. ^{[3
]}

Cairns, Timothy C. ^{[4
]}

Bignell, Elaine M. ^{[4
]}

Coutts, Robert H. A. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Nat Sci, Div Biol, London SW7 2AZ, England

[2] Natl Agr Res Ctr, Islamabad 45500, Pakistan

[3] Hammersmith Hosp, Fac Med, Div Infect Dis, London W12 0HS, England

[4] Univ London Imperial Coll Sci Technol & Med, Fac Med, Div Infect Dis, London SW7 2AZ, England

来源：

FUNGAL GENETICS AND BIOLOGY | 2011年 / 48卷 / 11期

关键词：

Aspergillus fumigatus; dsRNAs; Mycoviruses; Phenotype; Virulence; FUNGUS CRYPHONECTRIA-PARASITICA; VIRUS; CHRYSOVIRUS; PARTICLES; GENUS; PARTITIVIRUS; NIDULANS; FUSION; PCR;

D O I：

10.1016/j.fgb.2011.07.008

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Some isolates of the opportunistic human pathogenic fungus Aspergillus fumigatus are known to be infected with mycoviruses. The dsRNA genomes of two of these mycoviruses, which include a chrysovirus and a partitivirus, have been completely sequenced and an RT-PCR assay for the viruses has been developed. Through curing virus-infected A. fumigatus isolates by cycloheximide treatment and transfecting virus-free isolates with purified virus, as checked by RT-PCR, isogenic virus-free and virus-infected lines of the fungus were generated whose phenotypes and growth have been directly compared. Mycovirus infection of A. fumigatus with either the chrysovirus or the partitivirus resulted in significant aberrant phenotypic alterations and attenuation of growth of the fungus but had no effect on susceptibility to common antifungals. Chrysovirus infection of A. fumigatus caused no significant alterations to murine pathogenicity. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：1071 / 1075

页数：5

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