Hydrogen production by immobilized Synechocystis sp PCC 6803

被引：58

作者：

Touloupakis, Eleftherios ^{[1
]}

Rontogiannis, George ^{[2
]}

Silva Benavides, Ana Margarita ^{[3
,4
]}

Cicchi, Bernardo ^{[1
]}

Ghanotakis, Demetrios F. ^{[2
]}

Torzillo, Giuseppe ^{[1
]}

机构：

[1] CNR, Ist Studio Ecosistemi, Via Madonna Piano 10, I-50019 Sesto Fiorentino, Italy

[2] Univ Crete, Dept Chem, Voutes Heraklion 71003, Greece

[3] Univ Costa Rica, Escuela Biol, San Jose 2060, Costa Rica

[4] Univ Costa Rica, Ctr Invest Ciencias Mar & Limnol CIMAR, San Jose 2060, Costa Rica

来源：

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2016年 / 41卷 / 34期

关键词：

Synechocystis sp PCC 6803; Alginate; Immobilization; Biohydrogen; CHLAMYDOMONAS-REINHARDTII; GLOEOCAPSA-ALPICOLA; CYANOBACTERIAL HYDROGENASES; BIDIRECTIONAL HYDROGENASE; H-2; PRODUCTION; PHOTOPRODUCTION; GROWTH; CULTURES; REMOVAL; MEDIA;

D O I：

10.1016/j.ijhydene.2016.07.075

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The ability of the immobilized Synechocystis sp. PCC 6803 cells to produce hydrogen via an indirect light driven process was investigated. Cells were successfully immobilized in a calcium alginate gel matrix. Beads were prepared by using 3% (w/v) alginate and 2% (w/v) calcium chloride, and were stable showing minimal cell leakage. Beads had a mean diameter of 5.06 +/- 0.18 mm, a volume of 67.8 +/- 2.4 mu L and a mean cell dry weight of 46.3 +/- 0.8 mu g. Immobilized cells retained their viability, as shown by measurement of the F-v/F-m ratio, for at least 30 days. The maximum rate of hydrogen production reached 5.73 +/- 0.69 mL H-2 mg cells(-1) (e.g., 40.6 +/- 4.9 mu mol H-2 mg chl(-1) h(-1)). (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

引用

页码：15181 / 15186

页数：6

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