Simultaneous detection of glutamic acid decarboxylase and reelin mRNA in adult rat neurons using in situ hybridization and immunofluorescence

被引：23

作者：

Pesold, C

Pisu, MG

Impagnatiello, F

Uzunov, DP

Caruncho, HJ

机构：

[1] Univ Illinois, Coll Med, Dept Psychiat, Inst Psychiat, Chicago, IL 60612 USA

[2] Univ Santiago de Compostela, Dept Fundamental Biol, Galicia, Spain

来源：

BRAIN RESEARCH PROTOCOLS | 1998年 / 3卷 / 02期

关键词：

reelin; neurotrophic factor; in situ hybridization; immunocytochemistry;

D O I：

10.1016/S1385-299X(98)00036-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of in situ hybridization and immunocytochemical technique is an important tool to detail the biochemical phenotype of individual neurons. In this work, we have developed a double fluorescence method to show the presence of reelin mRNA in GABAergic cells. This was achieved by demonstrating the colocalization of glutamic acid decarboxylase(67), the synthesizing enzyme for GABA, with the mRNA for reelin, a novel factor involved in brain development and possibly the maintenance of the synaptic organization of layered structures in adult brain. The results demonstrated that reelin is expressed primarily in GABAergic cells in the adult rat cerebrum, but not in the cerebellum. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：155 / 160

页数：6

共 12 条

[1]

BRAHIC M, 1992, IN SITU HYBRIDIZATIO, P85

[2] A PROTEIN RELATED TO EXTRACELLULAR-MATRIX PROTEINS DELETED IN THE MOUSE MUTANT REELER [J].