A practical kit for micro-scale application of the ceiling crystallisation method

被引：7
|
作者：
Adawy, Alaa [1 ,3 ]
Corbeek, Wil [1 ]
de Ronde, Erik [1 ]
van Enckevort, Willem J. P. [1 ]
de Grip, Willem J. [2 ]
Vlieg, Elias [1 ]
机构：
[1] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 ED Nijmegen, Netherlands
[2] Radboud Univ Nijmegen, Med Ctr, Radboud Inst Mol Life Sci, Dept Biochem, NL-6525 ED Nijmegen, Netherlands
[3] Ain Shams Univ, Fac Sci, Dept Phys, Cairo, Egypt
来源：
CRYSTENGCOMM | 2015年 / 17卷 / 13期
关键词：
PROTEIN CRYSTAL-GROWTH; MARANGONI CONVECTION; DIFFUSION; NUCLEATION; GEOMETRY;
D O I：
10.1039/c4ce01814a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We designed a kit to facilitate the optimisation of protein crystal growth by means of the ceiling crystallisation method. This ceiling kit allows for diffusion-limited crystal growth with a total volume down to 10 mu l per trial and air-tight sealing for any type of protein sample. The ceiling crystals grow to sufficient size for diffraction experiments. In this highlight, we fully describe the features of this kit and how easily it can be used to yield protein crystals.
引用
收藏
页码：2602 / 2605
页数：4
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