Cross-amplified microsatellites in the European cherry fly, Rhagoletis cerasi: medium polymorphic-highly informative markers

被引：12

作者：

Augustinos, A. A. ^{[1
,2
,3
]}

Asimakopoulou, A. K. ^{[3
]}

Papadopoulos, N. T. ^{[4
]}

Bourtzis, K. ^{[1
]}

机构：

[1] Univ Ioannina, Dept Environm & Nat Resources Management, Agrinion 30100, Greece

[2] Univ Patras, Dept Biol, GR-26110 Patras, Greece

[3] Univ Thessaly, Dept Biochem & Biotechnol, Volos, Greece

[4] Univ Thessaly, Dept Agr Crop Prod & Agr Environm, Volos, Greece

来源：

BULLETIN OF ENTOMOLOGICAL RESEARCH | 2011年 / 101卷 / 01期

关键词：

Rhagoletis cerasi; Tephritidae; microsatellites; cross-species amplification; population analysis; MEDITERRANEAN FRUIT-FLY; POPULATION-STRUCTURE; OLIVE FLY; CERATITIS-CAPITATA; HOST RACES; DIPTERA; TEPHRITIDAE; LOCI; CALIFORNIA; EVOLUTION;

D O I：

10.1017/S0007485310000167

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The European cherry fruit fly, Rhagoletis cerasi (Diptera: Tephritidae), is a major pest of cherries in Europe and parts of Asia. Despite its big economic significance, there is a lack of studies on the genetic structure of its natural populations. Knowledge about an insect pest on molecular, genetic and population levels facilitates the development of environmentally friendly control methods. In this study, we present the development of 13 microsatellite markers for R. cerasi, through cross-species amplification. These markers have been used for the genotyping of 130 individuals from five different sampling sites in Greece. Our results indicate that (i) cross-species amplification is a versatile and rapid tool for developing microsatellite markers in Rhagoletis spp., (ii) the microsatellite markers presented here constitute an important tool for population studies on this pest, and (iii) there is clear structuring of natural European cherry fly populations.

引用

页码：45 / 52

页数：8

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