Second harmonic generation microscopy: principles and applications to disease diagnosis

被引：196
作者：
Campagnola, Paul J. [1 ]
Dong, Chen-Yuan [2 ,3 ]
机构：
[1] Univ Connecticut, Ctr Hlth, Dept Cell Biol, Ctr Cell Anal & Modeling, Farmington, CT 06030 USA
[2] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan
[3] Natl Taiwan Univ, Ctr Quantum Sci & Engn, Taipei 106, Taiwan
来源：
LASER & PHOTONICS REVIEWS | 2011年 / 5卷 / 01期
关键词：
Second harmonic generation; collagen; muscle; diseases; BASAL-CELL CARCINOMA; RAT-TAIL TENDON; EX-VIVO; IN-VIVO; MULTIPHOTON FLUORESCENCE; 2-PHOTON-EXCITED FLUORESCENCE; OSTEOGENESIS IMPERFECTA; STRUCTURAL ALTERATIONS; CONFOCAL MICROSCOPY; CORNEAL STROMA;
D O I：
10.1002/lpor.200910024
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this review, the authors discuss the underlying principles of SHG and specific factors that affect the generation properties and describe the essential components of a SHG instrument. In addition, results on the recent progress and impact SHG microscopy has made in different areas of biology and medicine are presented. In particular, the authors focus on disease diagnosis and basic research associated with connected tissues, musculo-skeletal disorders, and epithelial cancers. The presentation is concluded by offering a perspective on the future technical development of SHG microscopy and additional forefronts to be addressed. [GRAPHICS] SHG and multiphoton autofluorescence imaging of ex-vivo human dermis in 20 (left), 40 (middle), and 70 (right) years-old patients, where blue = SHG and green = autofluorescence.
引用
收藏
页码：13 / 26
页数：14
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