Silencing CD40 in dendritic cells by siRNA

被引：0

作者：

Qian, H ^{[1
]}

Li, M ^{[1
]}

Popov, IA ^{[1
]}

Ichim, TE ^{[1
]}

Zhong, R ^{[1
]}

Rycerz, K ^{[1
]}

Zeng, XF ^{[1
]}

Zhang, XS ^{[1
]}

Min, WP ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Surg Microbiol & Immunol, London, ON N6A 3K7, Canada

来源：

IMMUNOLOGY 2004: GENOMIC ISSUES, IMMUNE SYSTEM ACTIVATION AND ALLERGY | 2004年

关键词：

dendritic cells; gene silencing; RNA interference; siRNA; CD40;

D O I：

暂无

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have previously demonstrated that short interfering RNA (siRNA) is an effective method of gene-specific immune modulation in dendritic cells (DC) (J Immunol 171:691). In this study we investigated the feasibility of generating tolerogenic DC through silencing of CD40, one of the most important costimulatory molecules. Screening of effective siRNA specific to murine CD40 sequence was performed using the novel strategy of siRNA expression cassettes (SEC). Amongst the 4 designed SECs targeting the CD40 sequence (CD40-SEC), two SEC completely and partially suppressed CD40 expression on mature DCs. Functionally, CD40-SEC inhibited allostimulatory activity of DC in mixed lymphocyte culture (MLR). These data suggest that CD40-SEC is a useful tool for generating tolerogenic DC.

引用

页码：339 / 348

页数：10

共 26 条

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