miR-199b-5p inhibits triple negative breast cancer cell proliferation, migration and invasion by targeting DDR1

被引：33

作者：

Wu, Anhao ^{[1
]}

Chen, Yan ^{[2
]}

Liu, Yang ^{[1
]}

Lai, Yafang ^{[3
]}

Liu, Dequan ^{[1
]}

机构：

[1] Kunming Med Univ, Tumor Hosp Yunnan Prov, Affiliated Hosp 3, Dept Breast Surg Ward 1, 519 Kunzhou Rd, Kunming 650100, Yunnan, Peoples R China

[2] Kunming Med Univ, Tumor Hosp Yunnan Prov, Affiliated Hosp 3, Canc Inst, Kunming 650100, Yunnan, Peoples R China

[3] Guizhou Med Univ, Publ Hlth, Guiyang 550004, Guizhou, Peoples R China

来源：

ONCOLOGY LETTERS | 2018年 / 16卷 / 04期

关键词：

microRNA; discoidin domain receptor tyrosine kinase 1; microRNA-199b-5p; triple negative breast cancer; DISCOIDIN DOMAIN RECEPTOR-1; TYROSINE KINASE; HEPATOCELLULAR-CARCINOMA; POOR-PROGNOSIS; OVARIAN-CANCER; OVEREXPRESSION; ASSOCIATION; EXPRESSION;

D O I：

10.3892/ol.2018.9255

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Triple negative breast cancer (TNBC) has received increasing attention from oncologists worldwide due to its poor prognosis and paucity of targeted therapies. MicroRNAs (miRs) are a group of small non-coding RNAs that are responsible for the post-transcriptional regulation of various target genes. The present study demonstrated that the expression of miR-199b-5p in breast cancer tissue was significantly reduced compared with that in normal breast tissues by reverse transcription-quantitative polymerase chain reaction. In addition, western blot analysis and luciferase reporter assays revealed that miR-199b-5p in TNBC cells inhibited discoidin domain receptor tyrosine kinase 1 expression by directly targeting its 3-untranslated region. Furthermore, miR-199b-5p markedly suppressed the proliferation and invasion of TNBC cells, as demonstrated by using wound-healing, migration, invasion and proliferation assays. Collectively, these results indicate that miR-199b-5p may be a novel alternative therapeutic target for TNBC.

引用

收藏

页码：4889 / 4896

页数：8

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