Rapid and specific identification of Brucella abortus using the loop-mediated isothermal amplification (LAMP) assay

被引：17

作者：

Kang, Sung-Il ^{[1
]}

Her, Moon ^{[1
]}

Kim, Ji-Yeon ^{[1
]}

Lee, Jin Ju ^{[1
]}

Lee, Kichan ^{[1
]}

Sung, So-Ra ^{[1
]}

Jung, Suk Chan ^{[1
]}

机构：

[1] Anim & Plant Quarantine Agcy, Div Bacterial Dis, OIE Reference Lab Brucellosis, Anyang 430757, Gyeonggi Do, South Korea

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2015年 / 40卷

关键词：

Brucella abortus; Bst polymerase; Diagnosis; LAMP; Zoonosis; DIFFERENT PCR METHODS; SPP; BLOOD; DIFFERENTIATION; MELITENSIS; DNA;

D O I：

10.1016/j.cimid.2015.03.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A rapid and accurate diagnosis of brucellosis is required to reduce and prevent the spread of disease among animals and the risk of transfer to humans. In this study, a Brucella abortus-specific (Ba) LAMP assay was developed, that had six primers designed from the BruAb2_0168 region of chromosome I. The specificity of this LAMP assay was confirmed with Brucella reference strains, B. abortus vaccine strains, B. abortus isolates and phylogenetically or serologically related strains. The detection limit of target DNA was up to 20 fg/mu l within 60 min. The sensitivity of the new LAMP assay was equal to or slightly higher than other PCR based assays. Moreover, this Ba-LAMP assay could specifically amplify all B. abortus biovars compared to previous PCR assays. To our knowledge, this is the first report of specific detection of B. abortus using a LAMP assay. The Ba-LAMP assay can offer a rapid, sensitive and accurate diagnosis of bovine brucellosis in the field. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：1 / 6

页数：6

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