Flavobacterium amniphilum sp nov., isolated from a stream

Strain KYPY10(T), isolated from a water sample taken from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain KYPY10(T) were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped surrounded by a thick capsule, and formed light-yellow colonies. Growth occurred at 20-30 degrees C (optimum, 25 degrees C), at pH 6-7 (optimum, pH 6) and with 0-0.2% NaCl (optimum, 0% w/v). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain KYPY10(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium brevivitae TTM-43(T) (98.4% sequence similarity) and Flavobacterium vireti THG-SM1(T) (98.0 %). Strain KYPY10(T) contained iso-C-15:0, iso-C-15:1 G, summed feature 9 (iso-C-17:1 omega 9c and/or 10-methyl C-16:0), iso-C-17:0 3-OH, iso-C-15:0 3-OH and iso-C-16:0 as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine, four uncharacterized aminophospholipids, five uncharacterized phospholipids and one uncharacterized lipid. The major polyamines were homospermidine and putrescine. The genomic DNA G+C content of strain KYPY10(T) was 41.0 mol%. The DNA-DNA relatedness of strain KYPY10(T) with respect to recognized species of the genus Flavobacterium was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain KYPY10(T) was recognized as a representative of a novel species within the genus Flavobacterium. The name Flavobacterium amniphilum sp. nov. is proposed, with strain KYPY10(T) (= BCRC 81006(T) = LMG 29727(T) = KCTC 52443(T)) as the type strain.