Simultaneous depletion of albumin and immunoglobulin G by using twin affinity magnetic nanotraps

被引：9

作者：

Buyuktiryaki, Sibel ^{[1
]}

Uzun, Lokman ^{[2
]}

Denizli, Adil ^{[2
]}

Say, Ridvan ^{[3
]}

Ersoz, Arzu ^{[3
]}

机构：

[1] Anadolu Univ, BIBAM Plant Drug & Sci Res Ctr, Eskisehir, Turkey

[2] Hacettepe Univ, Dept Chem, Ankara, Turkey

[3] Anadolu Univ, Dept Chem, Eskisehir, Turkey

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2016年 / 51卷 / 12期

关键词：

Albumin depletion; IgG depletion; dye-ligand affinity chromatography; protein a affinity chromatography; covalent conjugation; HUMAN SERUM; CIBACRON BLUE; INTERACTION CHROMATOGRAPHY; MASS-SPECTROMETRY; STATIONARY-PHASE; PROTEIN-G; ADSORPTION; DYE; SEPARATION; PLASMA;

D O I：

10.1080/01496395.2016.1200086

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

For biomarker discovery, simultaneous removal of albumin and immunoglobulin G (IgG) become more important for both concentrating other species including potential biomarkers and getting rid of their masking effect. In this study, we have proposed a covalent and photosensitive cross-linking conjugation of the biomolecules on nanostructures for the depletion of target proteins from aqueous solution and serum. The effect of concentration, pH, temperature and ionic strength on the adsorption of twin affinity nanotraps based on Cibacron Blue F3GA and Protein A were investigated. The efficiency of albumin and IgG depletion from human serum was performed using sodium dodecylsulfate-polyacrylamide gel electrophoresis.

引用

页码：2080 / 2089

页数：10

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