High prevalence of Panton-Valentine leukocidin positive, multidrug resistant, Methicillin-resistant Staphylococcus aureus strains circulating among clinical setups in Adamawa and Far North regions of Cameroon

被引:11
作者
Mohamadou, Mansour [1 ,2 ,3 ]
Essama, Sarah Riwom [2 ]
Essome, Marie Chantal Ngonde [3 ]
Akwah, Lillian [2 ,3 ]
Nadeem, Nudrat [1 ]
Kamga, Hortense Gonsu [4 ]
Sattar, Sadia [1 ]
Javed, Sundus [1 ]
机构
[1] COMSATS Univ Islamabad, Biosci Dept, Islamabad, Pakistan
[2] Univ Yaounde I, Dept Microbiol, Fac Sci, Yaounde, Cameroon
[3] Inst Med Res & Med Plants Studies, Ctr Res Hlth & Prior Pathol, Yaounde, Cameroon
[4] Univ Yaounde I, Dept Microbiol Haematol & Infect Dis, Fac Med & Biomed Sci, Yaounde, Cameroon
关键词
CASSETTE CHROMOSOME MEC; ANTIBIOTIC-RESISTANCE; MULTIPLEX PCR; SUSCEPTIBILITY; INFECTIONS; HOSPITALS; IDENTIFICATION; EPIDEMIOLOGY; ELEMENT;
D O I
10.1371/journal.pone.0265118
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Staphylococcus aureus (S. aureus) is one of the earliest pathogens involved in human infections, responsible for a large variety of pathologies. Methicillin was the first antibiotic used to treat infections due to S. aureus but infections due to Methicillin resistant Staphylococcus aureus (MRSA) originated from hospital settings. Later, severe infections due to MRSA without any contact with the hospital environment or health care workers arose. Prevalence of MRSA has shown an alarming increase worldover including Cameroon. This Cross-sectional study was designed to evaluate the occurrence of MRSA infections in five different, most frequented Hospitals in northern Cameroon. Socio demographic data was recorded through questionnaire and different clinical specimens were collected for bacterial isolation. Identification of S. aureus was confirmed via 16s rRNA amplification using S. aureus specific primers. Molecular characterisation was performed through mecA gene, Luk PV gene screening and SCCmec typing. A total of 380 S. aureus clinical isolates were obtained of which 202 (53.2%) were nonduplicate multidrug resistant isolates containing, 45.5% MRSA. Higher number of MRSA was isolated from pus (30.4%) followed by blood culture (18.5%), and urine (17.4%). Patients aged 15 to 30 years presented high prevalence of MRSA (30.4%). Majority isolates (97.8%) carried the mecA gene, PVL toxin screening indicated 53.3% isolates carried the lukPV gene. Based on PVL detection and clinical history, CA-MRSA represented 53.3% of isolates. SCCmec typing showed that the Type IV was most prevalent (29.3%), followed by type I (23.9%). Amongst MRSA isolates high resistance to penicillin (91.1%), cotrimoxazole (86.7%), tetracycline (72.2%), and ofloxacin (70.0%) was detected. Meanwhile, rifampicin, fusidic acid, lincomycin and minocycline presented high efficacy in bacterial control. This study revealed a high prevalence of MRSA among infections due to S. aureus in Northern Cameroon. All MRSA recorded were multidrug resistant and the prevalence of CA MRSA are subsequently increasing, among population.
引用
收藏
页数:16
相关论文
共 69 条
[1]   The association between pathogen factors and clinical outcomes in patients with Staphylococcus aureus bacteraemia in a tertiary hospital, Cape Town [J].
Abdulgader, Shima M. ;
van Rijswijk, Amike ;
Whitelaw, Andrew ;
Newton-Foot, Mae .
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2020, 91 :111-118
[2]   Methicillin-resistant Staphylococcus aureus (MRSA) isolated from chicken meat and giblets often produces staphylococcal enterotoxin B (SEB) in non-refrigerated raw chicken livers [J].
Abolghait, Said Kamal ;
Fathi, Amal Gamal ;
Youssef, Fatma Mohamed ;
Algammal, Abdelazeem Mohamed .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2020, 328
[3]   Methicillin-Resistant Staphylococcus aureus (MRSA): One Health Perspective Approach to the Bacterium Epidemiology, Virulence Factors, Antibiotic-Resistance, and Zoonotic Impact [J].
Algammal, Abdelazeem M. ;
Hetta, Helal F. ;
Alkhalifah, Dalal Hussien H. ;
Hozzein, Wael N. ;
Batiha, Gaber El-Saber ;
El Nahhas, Nihal ;
Mabrok, Mahmoud A. ;
Elkelish, Amr .
INFECTION AND DRUG RESISTANCE, 2020, 13 :3255-3265
[4]   Nosocomial infections caused by community-associated methicillin-resistant Staphylococcus aureus in Colombia [J].
Alvarez, Carlos Arturo ;
Yomayusa, Nancy ;
Leal, Aura Lucia ;
Moreno, Jaime ;
Mendez-Alvarez, Sebastian ;
Ibanez, Milciades ;
Vanegas, Natasha .
AMERICAN JOURNAL OF INFECTION CONTROL, 2010, 38 (04) :315-318
[5]   The History of Methicillin-Resistant Staphylococcus aureus in Brazil [J].
Andrade, Mariana Moreira ;
Luiz, Wilson Barros ;
da Silva Oliveira Souza, Rayane ;
Amorim, Jaime Henrique .
CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY, 2020, 2020
[6]  
[Anonymous], 2013, ANT RES THREATS US, P114
[7]   Molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from patients at a tertiary care hospital in Hyderabad, South India [J].
Archana, Ganapuram J. ;
Sinha, Akhauri Yash ;
Annamanedi, Madhavi ;
Asrith, Kandala Pavan ;
Kale, Satyajit B. ;
Kurkure, Nitin, V ;
Doijad, Swapnil P. ;
Nagamani, Kammili ;
Hegde, Nagendra R. .
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2020, 38 (02) :183-+
[8]   Developing questionnaires for educational research: AMEE Guide No. 87 [J].
Artino, Anthony R., Jr. ;
La Rochelle, Jeffrey S. ;
Dezee, Kent J. ;
Gehlbach, Hunter .
MEDICAL TEACHER, 2014, 36 (06) :463-474
[9]   Staphylococcic necrotizing pneumopathy due to Panton-Valentine leukocidin toxin with good outcome [J].
Barcelo, M. ;
Chauvet, E. ;
Boukhari, R. ;
Mbieleu, B. .
ARCHIVES DE PEDIATRIE, 2009, 16 (01) :32-36
[10]   A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V [J].
Boye, K. ;
Bartels, M. D. ;
Andersen, I. S. ;
Moller, J. A. ;
Westh, H. .
CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (07) :725-727