Fibrinolytic enzyme production from low-cost substrates by marine Bacillus subtilis: Process optimization and kinetic modeling

被引:23
作者
Pan, Shihan [1 ,2 ]
Chen, Guiguang [1 ,2 ]
Zeng, Jingjing [1 ,2 ]
Cao, Xiaoyan [1 ,2 ]
Zheng, Xin [1 ,2 ]
Zeng, Wei [1 ,2 ]
Liang, Zhiqun [1 ,2 ]
机构
[1] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China
[2] Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Guangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Bacillus subtilis; Fibrinolytic enzyme; Process optimization; Oxygen supply; Kinetics; ALKALINE PROTEASE PRODUCTION; N-ACETYLGLUCOSAMINE PRODUCTION; LACTIC-ACID FERMENTATION; STEP-WISE REGULATION; DISSOLVED-OXYGEN; ANTITHROMBOTIC THERAPY; SUBMERGED FERMENTATION; NATTOKINASE PRODUCTION; MEDIA OPTIMIZATION; BATCH CULTURE;
D O I
10.1016/j.bej.2018.11.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Enhanced fibrinolytic enzyme production was achieved by systematic process optimization, using marine Bacillus subtilis D21-8 under submerged batch fermentation. A low-cost medium containing 23.2 g/L soybean meal, 24.1 g/L cassava starch and 1.6 g/L CaCl2 was attained by single factor experiments combined with statistical methods. The environmental conditions, optimized in a 250 mL shake flask, were 34 degrees C, 220 rpm and initial pH 5.0-5.2. Scale-up experiments were performed in fermenters with different oxygen supply. The highest fibrinolytic activity of 3787 U/mL was obtained in 100 L pilot fermenter with aeration rate of 1.0 vvm, which is among the highest reported in literature in terms of fibrinolytic enzyme production from cheap substrates. Kinetic modeling of the batch fermentation processes in fermenters using Logistic and Luedeking-Piret equations enables obtaining an improved insight of process kinetics.
引用
收藏
页码:268 / 277
页数:10
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