The Pseudomonas aeruginosa whole genome sequence: A 20th anniversary celebration

被引：8

作者：

Brinkman, Fiona S. L. ^{[1
]}

Winsor, Geoffrey L. ^{[1
]}

Done, Rachel E. ^{[2
]}

Filloux, Alain ^{[3
]}

Francis, Vanessa I. ^{[4
]}

Goldberg, Joanna B. ^{[2
]}

Greenberg, E. Peter ^{[5
]}

Han, Kook ^{[6
]}

Hancock, Robert E. W. ^{[7
]}

Haney, Cara H. ^{[8
]}

Haeussler, Susanne ^{[9
]}

Klockgether, Jens ^{[10
]}

Lamont, Iain L. ^{[11
]}

Levesque, Roger C. ^{[12
]}

Lory, Stephen ^{[6
]}

Nikel, Pablo I. ^{[13
]}

Porter, Steven L. ^{[4
]}

Scurlock, Matthew W. ^{[4
]}

Schweizer, Herbert P. ^{[14
]}

Tuemmler, Burkhard ^{[10
]}

Wang, Meng ^{[15
]}

Welch, Martin ^{[15
]}

机构：

[1] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada

[2] Emory Univ, Sch Med, Dept Pediat,Emory Childrens Ctr Cyst Fibrosis & A, Div Pulm Allergy & Immunol Cyst Fibrosis & Sleep, Atlanta, GA USA

[3] Imperial Coll London, Dept Life Sci, MRC Ctr Mol Bacteriol & Infect, London, England

[4] Univ Exeter, Geoffrey Pope Bldg, Exeter, Devon, England

[5] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[6] Harvard Med Sch, Dept Microbiol, Boston, MA 02115 USA

[7] Univ British Columbia, Vancouver, BC, Canada

[8] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC, Canada

[9] Helmholtz Ctr Infect Res, Dept Mol Bacteriol, Braunschweig, Germany

[10] Hannover Med Sch, Klin Padiatr Pneumol Allergol & Neonatol, Hannover, Germany

[11] Univ Otago, Dept Biochem, Dunedin, New Zealand

[12] Univ Laval, Fac Med, Inst Biol Integrat & Syst IBIS, Pavillon Charles Eugene Marchand, Quebec City, PQ, Canada

[13] Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Lyngby, Denmark

[14] No Arizona Univ, Pathogen & Microbiome Inst, Flagstaff, AZ 86011 USA

[15] Univ Cambridge, Dept Biochem, Hopkins Bldg, Cambridge, England

来源：

ADVANCES IN MICROBIAL PHYSIOLOGY, VOL 79 | 2021年 / 79卷

关键词：

GRAM-NEGATIVE BACTERIA; MINI-TN7; INSERTION; VIRULENCE FACTORS; OUTER-MEMBRANE; MUTANT LIBRARY; SMALL RNAS; IDENTIFICATION; EXPRESSION; DIVERSITY; GENES;

D O I：

10.1016/bs.ampbs.2021.07.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Toward the end of August 2000, the 6.3 Mbp whole genome sequence of Pseudomonas aeruginosa strain PAO1 was published. With 5570 open reading frames (ORFs), PAO1 had the largest microbial genome sequenced up to that point in time-including a large proportion ofmetabolic, transport and antimicrobial resistance genes supporting its ability to colonize diverse environments. A remarkable 9% of its ORFs were predicted to encode proteins with regulatory functions, providing new insight into bacterial network complexity as a function of network size. In this celebratory article, we fast forward 20 years, and examine how access to this resource has transformed our understanding of P. aeruginosa. What follows is more than a simple review or commentary; we have specifically asked some of the leaders in the field to provide personal reflections on how the PAO1 genome sequence, along with the Pseudomonas Community Annotation Project (PseudoCAP) and Pseudomonas Genome Database (pseudomonas.com), have contributed to the many exciting discoveries in this field. In addition to bringing us all up to date with the latest developments, we also ask our contributors to speculate on how the next 20 years of Pseudomonas research might pan out.

引用

页码：25 / 88

页数：64