Polyacrylamide-based monolithic capillary column with coating of cellulose tris(3,5-dimethylphenyl-carbamate) for enantiomer separation in capillary electrochromatography

被引：42
作者：
Dong, Xiaoli [1 ,2 ]
Wu, Ren'an [1 ]
Dong, Jing [1 ]
Wu, Minghuo [1 ]
Zhu, Yan [2 ]
Zou, Hanfa [1 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
[2] Zhejiang Univ, Dept Chem, Hangzhou, Peoples R China
来源：
ELECTROPHORESIS | 2008年 / 29卷 / 04期
关键词：
capillary electrochromatography; cellulose tris(3,5-dimethylphenyl-carbamate); enantioseparation; polyacrylamide; monolithic capillary column;
D O I：
10.1002/elps.200700644
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A hydrophilic chiral capillary monolithic column for enantiomer separation in CEC was prepared by coating cellulose tris(3,5-dimethylphenyl-carbamate) (CDMPC) on porous hydrophilic poly(acrylamide-co-N,N'-methylene-bisacrylamide) (poly(AA-co-MBA)) monolithic matrix with confine of a fused-silica capillary. The coating conditions were optimized to obtain a stable and reproducible chiral stationary phase for CEC. The effect of organic modifier of ACN in aqueous mobile phase for the enantiomer separation by CEC was investigated, and the significant influence of ACN on the enantioresolution and electrochromatographic retention was observed. Twelve pairs of enantiomers including acidic, neutral, and basic analytes were tested and nine pairs of them were baseline-enantioresolved with acidic and basic aqueous mobile phases. A good within-column repeatability in retention time (RSD = 2.4%) and resolution (RSD = 3.2%) was obtained by consecutive injections of a neutral compound, benzoin, on a prepared chiral monolithic column, while the between-column repeatability in retention time (RSD = 6.4%) and resolution (RSD = 9.6%) was observed by column-to-column examination. The prepared monolithic stationary phase showed good stability in either acidic or basic mobile phase.
引用
收藏
页码：919 / 927
页数：9
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