Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications

被引:21
作者
Bunge, Frank [1 ,2 ]
van den Driesche, Sander [1 ,2 ]
Vellekoop, Michael J. [1 ,2 ]
机构
[1] Univ Bremen, Inst Microsensors Actuators & Syst IMSAS, D-28359 Bremen, Germany
[2] Univ Bremen, MCB, D-28359 Bremen, Germany
关键词
Lab-on-a-Chip; hydrogel; cell cultivation; MDCK; diffusion model; parylene; CULTURE SYSTEM; OXYGEN SENSORS; SHEAR-STRESS;
D O I
10.3390/s17071603
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Lab-on-a-Chip (LoC) applications for the long-term analysis of mammalian cells are still very rare due to the lack of convenient cell cultivation devices. The difficulties are the integration of suitable supply structures, the need of expensive equipment like an incubator and sophisticated pumps as well as the choice of material. The presented device is made out of hard, but non-cytotoxic materials (silicon and glass) and contains two vertical arranged membranes out of hydrogel. The porous membranes are used to separate the culture chamber from two supply channels for gases and nutrients. The cells are fed continuously by diffusion through the membranes without the need of an incubator and low requirements on the supply of medium to the assembly. The diffusion of oxygen is modelled in order to find the optimal dimensions of the chamber. The chip is connected via 3D-printed holders to the macroscopic world. The holders are coated with Parlyene C to ensure that only biocompatible materials are in contact with the culture medium. The experiments with MDCK-cells show the successful seeding inside the chip, culturing and passaging. Consequently, the presented platform is a step towards Lab-on-a-Chip applications that require long-term cultivation of mammalian cells.
引用
收藏
页数:15
相关论文
共 37 条
[31]   On-chip multi-gas incubation for microfluidic cell cultures under hypoxia [J].
Takano, Atsushi ;
Tanaka, Masato ;
Futai, Nobuyuki .
BIOMICROFLUIDICS, 2014, 8 (06)
[32]   A novel 3D mammalian cell perfusion-culture system in microfluidic channels [J].
Toh, Yi-Chin ;
Zhang, Chi ;
Zhang, Jing ;
Khong, Yuet Mei ;
Chang, Shi ;
Samper, Victor D. ;
van Noort, Danny ;
Hutmacher, Dietmar W. ;
Yu, Hanry .
LAB ON A CHIP, 2007, 7 (03) :302-309
[33]   Three-dimensional co-cultures of human endothelial cells and embryonic stem cell-derived pericytes inside a microfluidic device [J].
van der Meer, Andries D. ;
Orlova, Valeria V. ;
ten Dijke, Peter ;
van den Berg, Albert ;
Mummery, Christine L. .
LAB ON A CHIP, 2013, 13 (18) :3562-3568
[34]   Commercialization of microfluidic devices [J].
Volpatti, Lisa R. ;
Yetisen, Ali K. .
TRENDS IN BIOTECHNOLOGY, 2014, 32 (07) :347-350
[35]   Phaseguides: a paradigm shift in microfluidic priming and emptying [J].
Vulto, Paul ;
Podszun, Susann ;
Meyer, Philipp ;
Hermann, Carsten ;
Manz, Andreas ;
Urban, Gerald A. .
LAB ON A CHIP, 2011, 11 (09) :1596-1602
[36]   The rate of oxygen utilization by cells [J].
Wagner, Brett A. ;
Venkataraman, Sujatha ;
Buettner, Garry R. .
FREE RADICAL BIOLOGY AND MEDICINE, 2011, 51 (03) :700-712
[37]   Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device [J].
Yu, Ting ;
Guo, Zhe ;
Fan, Hui ;
Song, Jing ;
Liu, Yuanbin ;
Gao, Zhancheng ;
Wang, Qi .
ONCOTARGET, 2016, 7 (18) :25593-25603