Characterization of hydrogels prepared from copolymerization of the different degrees of methacrylate-grafted chondroitin sulfate macromers and acrylic acid

被引:11
作者
Tsai, Ming-Fung [1 ]
Tsai, Hung-Yu [1 ]
Peng, Yu-Shiang [1 ]
Wang, Li-Fang [1 ]
Chen, Jenn-Shing [2 ]
Lu, Shui Chun [3 ]
机构
[1] Kaohsiung Med Univ, Sch Life Sci, Fac Med & Appl Chem, Kaohsiung 80708, Taiwan
[2] Natl Univ Kaohsiung, Dept Appl Chem, Kaohsiung, Taiwan
[3] Kaohsiung Med Univ, Dept Clin Res, Kaohsiung 80708, Taiwan
关键词
chondroitin sulfate; polyacrylic acid; methacrylation; hydrogels; cytotoxicity;
D O I
10.1002/jbm.a.31441
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Different degrees (between 20 and 75%) of methacrylate-grafted chondroitin sulfate (CS-MA) were synthesized. These CS-MA macromers were further copolymerized with acrylic acid (AA) at the molar ratio of 1 to 5 to form hydrogels. The sol percents of these CS-MA-AA hydrogels decreased and the cross-linking densities were studied with respect to the degrees of MA substitution onto CS-MA. The cytotoxicity with the increase in degree of MA substitution (DS) onto CS-MA as well as their hydrogels prepared from the corresponding macromers was tested using 293T cells. The cell viability of human dermal fibroblast and mescenchymal stem cells was further tested upon exposure to 75% CS-MA for 1-, 3-, and 7-day incubation period. The hydrogels maintained degradability for long periods of time as evidenced by SEM. A model protein, BSA, demonstrated the prolong-release behaviors of these hydrogels in simulated gastric fluids and pH 7.4 phosphate buffer solutions and a faster release rate in the presence of chondroitinase and esterase at pH 7.4. (C) 2007 Wiley Periodicals, Inc. J Biomed Mater Res.
引用
收藏
页码:727 / 739
页数:13
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