Protease encoding microbial communities and protease activity of the rhizosphere and bulk soils of two maize lines with different N uptake efficiency

被引：40

作者：

Baraniya, Divyashri ^{[1
]}

Puglisi, Edoardo ^{[2
]}

Ceccherini, Maria Teresa ^{[1
]}

Pietramellara, Giacomo ^{[1
]}

Giagnoni, Laura ^{[1
]}

Arenella, Mariarita ^{[1
]}

Nannipieri, Paolo ^{[1
]}

Renella, Giancarlo ^{[1
]}

机构：

[1] Univ Florence, Dept Agrifood Prod & Environm Sci, Piazzale Cascine 28, I-50144 Florence, Italy

[2] Univ Cattolica Sacro Cuore, Fac Sci Agr Alimentari & Ambientali, Ist Microbiol, Via Emilia Parmense 84, I-29122 Piacenza, Italy

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2016年 / 96卷

关键词：

Nitrogen use efficiency; Rhizosphere; Protease activity; Protease encoding genes; Illumina sequencing; PCR-DGGE; NITROGEN USE EFFICIENCY; GENES; DIVERSITY; MODEL;

D O I：

10.1016/j.soilbio.2016.02.001

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

This study was carried out to understand the interplay of plant Nitrogen Utilizing Efficiency (NUE) with protease activity and microbial proteolytic community composition in the rhizosphere and bulk soils. Protease activity, diversity and abundance of protease genes (using DGGE and qPCR respectively of two key bacterial protease encoding genes: alkaline metallo-peptidase (apr) and neutral-metallopeptidases (npr)) were monitored in both rhizosphere and bulk soils from two maize in-bred lines L05 and T250 with higher and lower NUE respectively, using a rhizobox approach. Illumina sequencing was employed to assess the diversity of proteolytic communities encoding for the above-mentioned protease genes. Our results show higher enzyme activity, higher abundance and diversity of proteolytic genes in L05 maize rhizosphere, with higher NUE than in T250 maize rhizosphere. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：176 / 179

页数：4

共 34 条

[1] Plant genotype strongly modifies the structure and growth of maize rhizosphere microbial communities [J].