Multi-plane, wide-field fluorescent microscopy for biodynamic imaging in vivo

被引：30
|
作者：
Shi, Ruheng [1 ]
Jin, Cheng [1 ]
Xie, Hao [2 ]
Zhang, Yuanlong [2 ]
Li, Xinyang [2 ,3 ]
Dai, Qionghai [2 ]
Kong, Lingjie [1 ,4 ]
机构：
[1] Tsinghua Univ, Dept Precis Instrument, State Key Lab Precis Measurement Technol & Instru, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Dept Automat, Beijing 100084, Peoples R China
[3] Tsinghua Univ, Grad Sch Shenzhen, Shenzhen 518055, Guangdong, Peoples R China
[4] Tsinghua Univ, IDG McGovern Inst Brain Res, Beijing 100084, Peoples R China
来源：
BIOMEDICAL OPTICS EXPRESS | 2019年 / 10卷 / 12期
基金：
中国国家自然科学基金;
关键词：
STRUCTURED ILLUMINATION MICROSCOPY; RESOLUTION; ORIENTATION; LIGHT; LIMIT;
D O I：
10.1364/BOE.10.006625
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Wide-field fluorescent microscopy (WFFM) is widely employed in biomedical studies, due to its inherent advantages in high-speed imaging of biological dynamics noninvasively and specifically. However, WFFM suffers from the loss of axial resolution and the poor resistance to light scattering in deep tissue imaging. Here we propose a novel WFFM which has the capability in optical sectioning and volumetric imaging. We perform speckle illumination with a digital-micromirror-device for optical sectioning and employ an electrically tunable lens for defocusing modulation so as to quickly switch the image planes. We demonstrate its applications in multi-plane, wide-field imaging of biological dynamics in both zebrafish brains and mouse brains in vivo. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：6625 / 6635
页数：11
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