Two-photon multiplexing bio-imaging using a combination of Eu- and Tb-bioprobes

被引：36

作者：

Placide, Virginie ^{[1
]}

Bui, Anh Thy ^{[1
]}

Grichine, Alexei ^{[2
,3
]}

Duperray, Alain ^{[2
,3
]}

Pitrat, Delphine ^{[1
]}

Andraud, Chantal ^{[1
]}

Maury, Olivier ^{[1
]}

机构：

[1] Univ Lyon, ENS Lyon, CNRS, Lab Chim UMR UMR 5182, F-69364 Lyon, France

[2] INSERM, IAB, F-38000 Grenoble, France

[3] Univ Grenoble Alpes, IAB, F-38000 Grenoble, France

来源：

DALTON TRANSACTIONS | 2015年 / 44卷 / 11期

关键词：

EFFICIENT 2-PHOTON-SENSITIZED LUMINESCENCE; EUROPIUM COMPLEXES; ENERGY-TRANSFER; CROSS-SECTIONS; SENSITIZED LUMINESCENCE; LANTHANIDE LUMINESCENCE; EXCITATION; MICROSCOPY; EMISSION; PROBES;

D O I：

10.1039/c4dt03115f

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

During the course of our research studies aiming to combine the unique photophysical properties of lanthanide complexes and the intrinsic advantage of a nonlinear two-photon excitation, we described the proof-of-concept of a biphotonic multiplexing bio-imaging experiment. To that end, a new Tb-luminescent bioprobe [TbL2] was designed using the TACN-trispicolinate platform. The biphotonic imaging of T24 fixed cells stained either with [TbL2] or with the already described [EuL1] complexes is reported. Finally the biphotonic multiplexing experiment was performed using a combination of both probes using the spectral mode of the two-photon microscope.

引用

页码：4918 / 4924

页数：7

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