Engineered cartilage regeneration from adipose tissue derived-mesenchymal stem cells: A morphomolecular study on osteoblast, chondrocyte and apoptosis evaluation

被引：35

作者：

Szychlinska, Marta Anna ^{[1
]}

Castrogiovanni, Paola ^{[1
]}

Nsir, Houda ^{[2
]}

Di Rosa, Michelino ^{[3
]}

Guglielmino, Claudia ^{[1
]}

Parenti, Rosalba ^{[4
]}

Calabrese, Giovanna ^{[4
]}

Pricoco, Elisabetta ^{[5
]}

Salvatorelli, Lucia ^{[5
]}

Magro, Gaetano ^{[5
]}

Imbesi, Rosa ^{[1
]}

Mobasheri, Ali ^{[6
,7
,8
,9
]}

Musumeci, Giuseppe ^{[1
,10
]}

机构：

[1] Univ Catania, Sch Med, Human Anat & Histol Sect, Dept Biomed & Biotechnol Sci, Catania, Italy

[2] Univ Carthage, Ctr Biotechnol Borj Cedreya, Biotechnol Lab Olive Tree, Carthage, Tunisia

[3] Univ Catania, Sch Med, Pathol Sect, Dept Biomed & Biotechnol Sci, Catania, Italy

[4] Univ Catania, Sch Med, Physiol Sect, Dept Biomed & Biotechnol Sci, Catania, Italy

[5] Univ Catania, Sch Med, Anat Pathol Sect, Dept Med & Surg Sci & Adv Technol, Catania, Italy

[6] Univ Surrey, Sch Vet Med,Fac Hlth & Med Sci, D BOARD European Consortium Biomarker Discovery, APPROACH Innovat Medicines Initiat IMI Consortium, Duke Kent Bldg, Guildford GU2 7XH, Surrey, England

[7] King Abdulaziz Univ, Ctr Excellence Genom Med Res, King Fahd Med Res Ctr, Jeddah 21589, Saudi Arabia

[8] Univ Nottingham, Arthrit Res UK Ctr Sport Exercise & Osteoarthriti, Arthrit Res UK Pain Ctr, MRC,Queens Med Ctr, Nottingham NG7 2UH, England

[9] Univ Nottingham, Arthrit Res UK Ctr Musculoskeletal Ageing Res, Queens Med Ctr, Nottingham NG7 2UH, England

[10] Inst Etud Univ, UniPoliSI, Dept Hlth, Veyras, Switzerland

来源：

EXPERIMENTAL CELL RESEARCH | 2017年 / 357卷 / 02期

关键词：

Adipose tissue; Apoptosis; Cartilage; Chondrocytes; Collagen cell carrier scaffold; Lubricin; Mesenchymal stem cells; Regeneration; RUNX2; ARTICULAR-CARTILAGE; LUBRICIN DISTRIBUTION; OSTEOARTHRITIS; EXPRESSION; IMMUNOLOCALIZATION; DEGENERATION; FRICTION; LIGAMENT; GROWTH; INJURY;

D O I：

10.1016/j.yexcr.2017.05.018

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The poor self-repair capacity of cartilage tissue in degenerative conditions, such as osteoarthritis (OA), has prompted the development of a variety of therapeutic approaches, such as cellular therapies and tissue engineering based on the use of mesenchymal stem cells (MSCs). The aim of this study is to demonstrate, for the first time, that the chondrocytes differentiated from rat adipose tissue derived-MSCs (AMSCs), are able to constitute a morphologically and biochemically healthy hyaline cartilage after 6 weeks of culture on a Collagen Cell Carrier (CCC) scaffold. In this study we evaluated the expression of some osteoblasts (Runt-related transcription factor 2 (RUNX2) and osteocalcin), chondrocytes (collagen I, II and lubricin) and apoptosis (caspase-3) biomarkers in undifferentiated AMSCs, differentiated AMSCs in chondrocytes cultured in monolayer and AMSCs-derived chondrocytes seeded on CCC scaffolds, by different techniques such as immunohistochemistry, ELISA, Western blot and gene expression analyses. Our results showed the increased expression of collagen II and lubricin in AMSCs-derived chondrocytes cultured on CCC scaffolds, whereas the expression of collagen I, RUNX2, osteocalcin and caspase-3 resulted decreased, when compared to the controls. In conclusion, this innovative basic study could be a possible key for future therapeutic strategies for articular cartilage restoration through the use of CCC scaffolds, to reduce the morbidity from acute cartilage injuries and degenerative joint diseases.

引用

页码：222 / 235

页数：14

共 48 条

[1] Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation [J].