Genetic diversity of piroplasms in plains zebra (Equus quagga burchellii) and Cape mountain zebra (Equus zebra zebra) in South Africa

被引：19

作者：

Bhoora, Raksha ^{[1
]}

Buss, Peter ^{[2
]}

Guthrie, Alan J. ^{[3
]}

Penzhorn, Barend L. ^{[1
]}

Collins, Nicola E. ^{[1
]}

机构：

[1] Univ Pretoria, Fac Vet Sci, Dept Vet Trop Dis, ZA-0110 Onderstepoort, South Africa

[2] Kruger Natl Pk, Vet Wildlife Serv, ZA-1350 Skukuza, South Africa

[3] Univ Pretoria, Fac Vet Sci, Equine Res Ctr, ZA-0110 Onderstepoort, South Africa

来源：

VETERINARY PARASITOLOGY | 2010年 / 174卷 / 1-2期

基金：

新加坡国家研究基金会;

关键词：

Equine piroplasmosis; Theileria equi; Babesia caballi; Zebra; 18S rRNA gene; Phylogenetic analysis; BABESIA-CABALLI; THEILERIA-EQUI; QUANTITATIVE DETECTION; SERODIAGNOSIS; PACKAGE; ASSAY;

D O I：

10.1016/j.vetpar.2010.08.014

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Seventy EDTA blood samples collected from plains zebra (Equus quagga burchellii) and Cape mountain zebra (Equus zebra zebra) were screened for the presence of piroplasm parasite DNA using quantitative T. equi-specific and B. caballi-specific TaqMan real-time PCR (qPCR) tests. T. equi parasite DNA was detected in 60 samples, 19 of which were also positive for B. caballi. Approximately 1480 bp of the piroplasm 18S rRNA gene was amplified and sequenced from 17 samples, while the V4 hypervariable region of the 18S rRNA gene was amplified, cloned and sequenced from 31 samples. BLASTN analysis revealed that all of the sequences obtained were most similar to T. equi genotypes and not B. caballi genotypes. Although Babesia parasites were present in some of these samples, as indicated by qPCR, the parasitaemia may have been too low to allow detection by cloning of PCR products from a mixed infection. Sequence analyses of both the full-length and the V4 hypervariable region of the T. equi 18S rRNA gene revealed the existence of 13 new T. equi sequences from zebra, confirming the existence of sequence heterogeneity in the rRNA genes of the parasites that cause equine piroplasmosis, and further suggesting that there may be additional, as yet unidentified, T. equi and B. caballi 18S rRNA sequences present in the horse and zebra populations in South Africa. The occurrence of previously unrecognized sequence variation could pose a potential problem in the implementation of diagnostic tests targeting the 185 rRNA gene. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：145 / 149

页数：5

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