Mucin/carbopol matrix to immobilize oxalate oxidase in a urine oxalate amperometric biosensor

被引：21

作者：

Capra, RH

Strumia, M

Vadgama, PM

Baruzzi, AM ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, INFIQC, Dept Fisicoquim, RA-5000 Cordoba, Argentina

[2] Univ Nacl Cordoba, Fac Ciencias Quim, IMBIV, Dept Quim Organ, RA-5000 Cordoba, Argentina

[3] Univ London, IRC Biomed Mat, London WC1E 7HU, England

来源：

ANALYTICA CHIMICA ACTA | 2005年 / 530卷 / 01期

关键词：

mucin; carbopol; oxalate biosensor; amperometric biosensor; oxalate oxidase;

D O I：

10.1016/j.aca.2004.08.037

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzymatic amperometric electrode with extended analytical range and improved stability for oxalate determination has been developed. Glutarlaldehyde/mucin/carbopol matrix was used for the crosslinking of the enzyme between polymeric membranes to form a classical laminate construction (sandwich) and compared with the glutaraldehyde/mucin/enzyme and glutaraldehyde/albumin/enzyme. The use of a sulphonated membrane as internal membrane allowed rejection of the most important electrooxidable urine interferents. The recovery assays were highly satisfactory. The wide linear response in the range 2-400 muM after 1/10 urine dilution (corresponding to 20-4000 muM) made it suitable for clinical range. High correlation with the standard spectrophotometric method was obtained (r(2) = 0.98, y 0.89x, n = 25). (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：49 / 54

页数：6

共 22 条

[1] RHEOLOGICAL PROPERTIES OF CARBOPOL GELS .2. OSCILLATORY PROPERTIES OF CARBOPOL GELS [J].