Regional assignment and tissue expression of twenty-three expressed sequence tags (ESTs) from human chromosome 5

被引：7
作者：
Feldblyum, TV
Maglott, DR
McPherson, JD
Adams, M
Apostol, BL
Durkin, AS
Wasmuth, JJ
Nierman, WC
机构：
[1] AMER TYPE CULTURE COLLECT,ROCKVILLE,MD 20852
[2] UNIV CALIF IRVINE,COLL MED,DEPT BIOL CHEM,IRVINE,CA 92717
[3] INST GENOM RES,ROCKVILLE,MD 20850
来源：
GENOMICS | 1996年 / 33卷 / 01期
关键词：
D O I：
10.1006/geno.1996.0169
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Regional localization and expression patterns are reported for 19 expressed sequence tags (ESTs) from human chromosome 5, two of which were derived from the same transcript. Two of the ESTs correspond to genes not previously characterized in humans: a stress-activated protein kinase and nicotinamide nucleotide transhydrogenase. Expression was determined by three methods: Northern blots, PCR from tissue-specific cDNA libraries, and sequence sampling from EST sequencing projects. Six of the ESTs show no expression, and EST01986 appears to be expressed predominantly in the brain by all methods tested. (C) 1996 Academic Press, Inc.
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收藏
页码：128 / 130
页数：3
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