Structure determination of an intercalating ruthenium dipyridophenazine complex which kinks DNA by semiintercalation of a tetraazaphenanthrene ligand

被引：120

作者：

Hall, James P. ^{[1
]}

O'Sullivan, Kyra ^{[2
,3
]}

Naseer, Abeer ^{[1
]}

Smith, Jayden A. ^{[2
,3
]}

Kelly, John M. ^{[2
,3
]}

Cardin, Christine J. ^{[1
]}

机构：

[1] Univ Reading, Dept Chem, Reading RG6 6AD, Berks, England

[2] Trinity Coll Dublin, Ctr Chem Synth & Chem Biol, Dublin 2, Ireland

[3] Trinity Coll Dublin, Sch Chem, Dublin 2, Ireland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 43期

基金：

爱尔兰科学基金会;

关键词：

DNA oligonucleotide; ruthenium complex; X-ray crystal structure; CRYSTAL-STRUCTURE; METAL-COMPLEXES; CROSS-LINK; BINDING; SEQUENCE; SPECTROSCOPY; DICHROISM; GROOVE;

D O I：

10.1073/pnas.1108685108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a crystal structure, at atomic resolution (1.1 angstrom, 100 K), of a ruthenium polypyridyl complex bound to duplex DNA, in which one ligand acts as a wedge in the minor groove, resulting in the 51 degrees kinking of the double helix. The complex cation Lambda-[Ru(1,4,5,8-tetraazaphenanthrene)(2)(dipyridophenazine)](2+) crystallizes in a 1: 1 ratio with the oligonucleotide d(TCGGCGCCGA) in the presence of barium ions. Each complex binds to one duplex by intercalation of the dipyridophenazine ligand and also by semi-intercalation of one of the orthogonal tetraazaphenanthrene ligands into a second symmetrically equivalent duplex. The result is noncovalent cross-linking and marked kinking of DNA.

引用

页码：17610 / 17614

页数：5

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