Molecular characterization of Clostridium tetani strains by pulsed-field gel electrophoresis and colony PCR

被引:10
|
作者
Plourde-Owobi, L [1 ]
Seguin, D [1 ]
Baudin, MA [1 ]
Moste, C [1 ]
Rokbi, B [1 ]
机构
[1] Aventis Pasteur SA, Res Dept, F-69280 Marcy Letoile, France
关键词
D O I
10.1128/AEM.71.9.5604-5606.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.
引用
收藏
页码:5604 / 5606
页数:3
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