Upstream process intensification and continuous manufacturing

被引：69

作者：

Chen, Chun ^{[1
]}

Wong, H. Edward ^{[1
]}

Goudar, Chetan T. ^{[1
]}

机构：

[1] Amgen Inc, Amgen Ctr Dr, Proc Dev, 1 Amgen Ctr Dr, Thousand Oaks, CA 91320 USA

来源：

CURRENT OPINION IN CHEMICAL ENGINEERING | 2018年 / 22卷

关键词：

MAMMALIAN-CELL CULTURE; INDUCED-DISSOCIATION SPECTRA; ALTERNATING TANGENTIAL FLOW; HAMSTER OVARY CELLS; IN-SITU MICROSCOPY; FED-BATCH PROCESS; DENSITY PERFUSION; DNA METHYLATION; PRODUCTIVITY; LINE;

D O I：

10.1016/j.coche.2018.10.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Manufacturing process of biopharmaceuticals usually starts from culturing recombinant protein producing cells, predominately Chinese Hamster Ovary (CHO) cells, in bioreactors. Currently, fed-batch cell culture process is the most common upstream process method due to its operational simplicity and industrial familiarity. Intensified cell culture process, enabled by perfusion and other technologies, offers many benefits over traditional fed-batch cell culture mode. In this review, we discuss the current state-of-art for upstream cell culture process intensification with a focus towards continuous manufacturing. We also explore key technical drivers and their roles in steering towards a more cost-effective and flexible upstream biomanufacturing.

引用

页码：191 / 198

页数：8

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