Characterization of OuterMembrane Vesicles from Brucella melitensis and Protection Induced in Mice

被引：54
作者：
Daniel Avila-Calderon, Eric [1 ]
Lopez-Merino, Ahide E. [1 ]
Jain, Neeta [2 ]
Peralta, Humberto [3 ]
Oliver Lopez-Villegas, Edgar [1 ]
Sriranganathan, Nammalwar [2 ]
Boyle, Stephen M. [2 ]
Witonsky, Sharon [2 ]
Contreras-Rodriguez, Araceli [1 ]
机构：
[1] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico
[2] Virginia Tech, Virginia Maryland Reg Coll Vet Med, Ctr Mol Med & Infect Dis, Blacksburg, VA 24060 USA
[3] Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Cuernavaca 62210, Mor, Mexico
来源：
CLINICAL & DEVELOPMENTAL IMMUNOLOGY | 2012年
关键词：
OUTER-MEMBRANE VESICLES; GRAM-NEGATIVE BACTERIA; ESCHERICHIA-COLI; DENDRITIC CELLS; ABORTUS INFECTION; PSEUDOMONAS-AERUGINOSA; STRESS RESISTANCE; IMMUNE-RESPONSE; BALB/C MICE; IN-VIVO;
D O I：
10.1155/2012/352493
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The outer membrane vesicles (OMVs) from smooth B. melitensis 16 M and a derived rough mutant, VTRM1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice. Proteomic analysis showed 29 proteins present in OMVs from B. melitensis 16 M; some of them are well-known Brucella immunogens such as SOD, GroES, Omp31, Omp25, Omp19, bp26, and Omp16. OMVs from a rough VTRM1 induced significantly higher expression of IL-12, TNF alpha, and IFN gamma genes in bone marrow dendritic cells than OMVs from smooth strain 16 M. Relative to saline control group, mice immunized intramuscularly with rough and smooth OMVs were protected from challenge with virulent strain B. melitensis 16 M just as well as the group immunized with live strain B. melitensis Rev1 (P < 0.005). Additionally, the levels of serum IgG2a increased in mice vaccinated with OMVs from rough strain VTRM1 consistent with the induction of cell-mediated immunity.
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页数：13
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