Low background signal platform for the detection of ATP: When a molecular aptamer beacon meets graphene oxide

被引:113
作者
He, Yue
Wang, Zhi-Gang
Tang, Hong-Wu [1 ]
Pang, Dai-Wen
机构
[1] Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ,Wuhan Inst Biotechnol, Coll Chem & Mol Sci,Res Ctr Nanobiol & Nanomed,MO, Wuhan 430072, Peoples R China
基金
中国国家自然科学基金;
关键词
Molecular aptamer beacon; Graphene oxide; Fluorescence quenching; ATP; RESONANCE ENERGY-TRANSFER; DNA APTAMER; SENSING BIOMOLECULES; CARBON NANOTUBES; FLUORESCENCE; SENSORS; ADENOSINE; SELECTION; ASSAY; RECOGNITION;
D O I
10.1016/j.bios.2011.07.069
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A novel molecular aptamer beacon (MAB) was designed by integrating a single-labeled hairpin-shaped aptamer and graphene oxide (GO). The hairpin-shaped aptamer was constructed with anti-ATP aptamer and another five nucleotides added to the 5'-end of the aptamer which are complementary to nucleotides at the 3'-end of the aptamer to form a hairpin-shaped probe. This newly designed MAB which acts as a low background signal platform was used for the ATP detection based on long-range resonance energy transfer (LrRET). In the absence of ATP, the adsorption of the dye-labeled hairpin-shaped aptamer on GO makes the dyes close proximity to GO surface resulting in high efficiency quenching of fluorescence of the dyes. Therefore, the fluorescence of the designed MAB is completely quenched by GO, and the system shows very low background. Conversely, and very importantly, upon the adding of ATP, the quenched fluorescence is recovered significantly, and ATP can be detected in a wide range of 5-2500 mu M with a detection limit of 2 mu M and good selectivity. Moreover, when the GO-based MAB was used in cellular ATP assays, preeminent fluorescence signals were obtained, thus the platform of GO-based MAB could be used to detect ATP in real-world samples. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:76 / 81
页数:6
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