Cd-Aptamer Electrochemical Biosensor Based on AuNPs/CS Modified Glass Carbon Electrode

被引：205

作者：

Liu, Yuan ^{[1
]}

Lai, Yuxuan ^{[1
]}

Yang, Gaojian ^{[1
]}

Tang, Congli ^{[1
]}

Deng, Yan ^{[1
,2
]}

Li, Song ^{[1
]}

Wang, Zunliang ^{[2
]}

机构：

[1] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Econ Forest Cultivat & Utilizat Collaborat Innova, Zhuzhou 412007, Peoples R China

[2] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2017年 / 13卷 / 10期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Aptamer; Electrochmical Biosensor; Cadmium; Differential Pulse Voltammetry (DPV); Detection; HEAVY-METAL IONS; GOLD NANOPARTICLES; MESOPOROUS SILICA; PASTE ELECTRODE; ACETYLCHOLINESTERASE BIOSENSOR; PLATINUM NANOPARTICLES; MAGNETIC NANOPARTICLES; RAPID DETECTION; DNA APTAMERS; CANCER-CELLS;

D O I：

10.1166/jbn.2017.2424

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

An ultrasensitive aptamer electrochemical biosensor based on glass carbon electrode (GCE) modified with chitosan (CS), gold nanoparticles (AuNPs) and aptamer has been successfully constructed and used to detect cadmium ions (Cd2+). The poly-(diallyl dimethyl ammonium chloride) (PDDA) was applied to neutralize Cd-aptamer via electrostatic interaction. The presence of Cd2+ would trigger Cd-aptamer conformation change causing the Cd/Cd-aptamer complex to adsorb more electrochemical signal indicator [Ru(NH3)(6)](3+) instead of PDDA. Through differential pulse voltammetry (DPV) detection, the peak current increased along with the increase in concentration of Cd2+, and the linear ranges were from 0.001 nM to 100 nM (1.124x10(-13) g/mL to 1.124x10(-8) g/mL) with the detection limit as low as 0.04995 pM (5.614x10(-15) g/mL). The proposed biosensor exhibited good stability, high sensitivity and selectivity towards Cd2+. Furthermore, this Cd-aptamer biosensor was demonstrated in testing Cd2+ in tap water samples with satisfactory results.

引用

页码：1253 / 1259

页数：7

共 69 条

[1] Ni0.5Zn0.5Fe2O4 nanocomposite modified carbon paste electrode for highly sensitive and selective simultaneous electrochemical determination of trace amounts of mercury (II) and cadmium (II) [J].