Secretion of TNF-α, IL-8 and nitric oxide by macrophages activated with Agaricus blazei Murill fractions in vitro

被引：87

作者：

Sorimachi, K ^{[1
]}

Akimoto, K

Ikehara, Y

Inafuku, K

Okubo, A

Yamazaki, S

机构：

[1] Dokkyo Univ, Sch Med, Dept Microbiol, Mibu, Tochigi 3210293, Japan

[2] Dokkyo Univ, Sch Med, Inst Med Res, Mibu, Tochigi 3210293, Japan

[3] Okinawa Fermentat Chem Co Ltd, Itoman, Okinawa 9010305, Japan

[4] Univ Tokyo, Dept Appl Biol Chem, Tokyo 1138657, Japan

来源：

CELL STRUCTURE AND FUNCTION | 2001年 / 26卷 / 02期

关键词：

Agaricus blazei Murill; macrophages; TNF-alpha; IL-8; nitric oxide (NO);

D O I：

10.1247/csf.26.103

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Water extracts of the mycelial culture and fruiting bodies of Agaricus blazei Murill were fractionated by ethanol precipitation using various ethanol concentrations. Original water extracts from mycelia (Fraction A-0) and fruiting bodies (Fraction B-0) induced TNF-alpha secretion by macrophages derived from rat bone marrow Fractions B-4 and B-5 obtained from ethanol precipitation of fruiting bodies using 44% and 50% ethanol, respectively, and Fraction B-6 obtained from the supernatant at 50% ethanol markedly induced TNF-a secretion. Similar effects were observed in IL-8 secretion by macrophages. Regarding nitric oxide (NO), Fraction B-5 induced a significant increase in NO secretion and Fractions B-4 and B-6 induced slightly NO secretion. Northern blot analysis showed that the increases in cytokine- and NO secretion were due to an increase in cytokine mRNAs or NO synthase mRNA, Therefore, it is concluded that Agaricus blazei Murill components which activate macrophages result in the induction of cytokine- and NO secretion in vitro.

引用

页码：103 / 108

页数：6

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