Engineering a biomimetic integrated scaffold for intervertebral disc replacement

被引：34

作者：

Du, Lilong ^{[1
]}

Yang, Qiang ^{[1
]}

Zhang, Jiamin ^{[2
]}

Zhu, Meifeng ^{[2
]}

Ma, Xinlong ^{[1
]}

Zhang, Yang ^{[3
]}

Wang, Lianyong ^{[2
]}

Xu, Baoshan ^{[1
]}

机构：

[1] Tianjin Hosp, Dept Minimally Invas Spine Surg, Tianjin 300211, Peoples R China

[2] Nankai Univ, Coll Life Sci, Minist Educ, Key Lab Bioact Mat, Tianjin 300071, Peoples R China

[3] Tianjin Orthoped Inst, Tianjin 300211, Peoples R China

来源：

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2019年 / 96卷

基金：

中国国家自然科学基金;

关键词：

Intervertebral disc; Wet spinning; Tissue engineering; Nucleus pulposus; Annulus fibrosus; ANNULUS FIBROSUS; REGENERATIVE MEDICINE; ELECTROSPUN SCAFFOLDS; NUCLEUS PULPOSUS; THERAPIES; DEGENERATION; MATRIX; REPAIR; CELLS; STRATEGIES;

D O I：

10.1016/j.msec.2018.11.087

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Tissue engineering technology provides a promising alternative to restore physiological functionality of damaged intervertebral disc (IVD). Advanced tissue engineering strategies for IVD have increasingly focused on engineering IVD regions combined the inner nucleus pulposus (NP) and surrounding annulus fibrosus (AF) tissue. However, simulating the cellular and matrix structures and function of the complex structure of IVD is still a critical challenge. Toward this goal, this study engineered a biomimetic AF-NP composite with circumferentially oriented poly(e-caprolactone) microfibers seeded with AF cells, with an alginate hydrogel encapsulating NP cells as a core. Fluorescent imaging and histological analysis showed that AF cells spread along the circumferentially oriented PCL microfibers and NP cells colonized in the alginate hydrogel similar to native IVD, without obvious migration and mixing between the AF and NP region. Engineered IVD implants showed progressive tissue formation over time after subcutaneous implantation in nude mice, which were indicated by deposition and organization of extracellular matrix and enhanced mechanical properties. In terms of form and function of IVD-like tissue, our engineered biomimetic AF-NP composites have potential application for IVD replacement.

引用

页码：522 / 529

页数：8

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