High concentrations of the macrophage migration inhibitory factor in human seminal plasma and prostatic tissues

被引：11
作者：
Frenette, G
Tremblay, RR
Dubé, JY
Lazure, C
Lemay, M
机构：
[1] CHU Laval, Res Ctr, Hormonal Bioregulat Lab, St Foy, PQ G1V 4G2, Canada
[2] Clin Res Inst Montreal, Lab Struct & Metab Neuropeptides, Montreal, PQ H2W 1R7, Canada
[3] CHU Laval, Dept Pathol, St Foy, PQ G1V 4G2, Canada
来源：
ARCHIVES OF ANDROLOGY | 1998年 / 41卷 / 03期
关键词：
cytokine; kallikrein hK2; proinflammatory mediator; prostatic pathology; prostatic secretion;
D O I：
10.3109/01485019808994890
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
During purification procedures to isolate kallikrein hK2 from human seminal plasma, kallikrein hK2 was found to be associated with another protein after several chromatographic steps. This study was conducted to identify the hK2 companion protein and characterize its properties and distribution. The protein was identified as macrophage migration inhibitory factor (MIF) by its NH2-terminal amino acid sequence. It had an enzymatic activity identical to that of recombinant MIF. Its concentration varied between 1 and 10 mu g/mL in various seminal plasma. By immunohistochemical analysis, MIF was found to be localized mainly in the epithelial cells of normal and cancerous prostates. Since MIF is a well-known proinflammatory mediator, these results suggest that it may have important functions in both human reproduction and prostatic physiology.
引用
收藏
页码：185 / 193
页数：9
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