Preparation of a Flower-Like Immobilized D-Psicose 3-Epimerase with Enhanced Catalytic Performance

被引：41

作者：

Zheng, Lu ^{[1
]}

Sun, Yining ^{[1
]}

Wang, Jing ^{[2
]}

Huang, He ^{[1
]}

Geng, Xin ^{[1
]}

Tong, Yi ^{[3
]}

Wang, Zhi ^{[1
]}

机构：

[1] Jilin Univ, Key Lab Mol Enzymol & Engn, Minist Educ, Coll Life Sci, Changchun 130012, Jilin, Peoples R China

[2] COFCO Corp, Nutr & Hlth Res Inst, Beijing 102209, Peoples R China

[3] Jilin COFCO Biochem Co Ltd, Natl Engn Res Ctr Corn Deep Proc, Changchun 130033, Jilin, Peoples R China

来源：

CATALYSTS | 2018年 / 8卷 / 10期

关键词：

nanoflower; immobilization; metal-dependent enzyme; D-psicose; DPEase; HYDROPHOBIC SUPPORTS; ENZYME-ACTIVITY; HYBRID NANOFLOWERS; THERMAL-STABILITY; COBALT PHOSPHATE; D-FRUCTOSE; LIPASES; SYSTEM; STABILIZATION; TEMPERATURE;

D O I：

10.3390/catal8100468

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this present study, we proposed a smart biomineralization method for creating hybrid organic-inorganic nanoflowers using a Co2+-dependent enzyme (D-psicose 3-epimerase; DPEase) as the organic component and cobalt phosphate as the inorganic component. The prepared nanoflowers have many separated petals that have a nanometer size. Under optimum conditions (60 degrees C and pH of 8.5), the nanoflower can display its maximum activity (36.2 U/mg), which is about 7.2-fold higher than free DPEase. Furthermore, the immobilized DPEase presents enhanced pH and thermal stabilities. The DPEase-nanoflower maintained about 90% of its activity after six reaction cycles, highlighting its excellent reusability.

引用

页数：10

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